Antimicrobial and Antioxidant Activitiesof Resins and Essential Oil From Pine (Pinus merkusii, Pinus oocarpa, Pinus insularis) and Agathis (Agathis loranthifolia)

  • Mardho Tillah Bogor Agricultural University, Bogor, Indonesia.
  • Irmanida Batubara Bogor Agricultural University, Indonesia.
  • Rita Kartika Sari Bogor Agricultural University, Indonesia

Abstract

This research aimed to explore the antibacterial  and antioxidant activities of Pinus merkusii, Pinus oocarpa, Pinus insularis, Agathis loranthifolia resins and essential oil. Resin was separated by distillation process to get essential oil and the residue was extracted using n-hexane, ethyl acetate (EtOAc), and methanol (MeOH). Antioxidant activity was performed by DPPH (1,1diphenyl-2-picryl hydrazyl) radical scavenging method. The antibacterial activity of resins and essential oil of the samples  determined using the disc diffusion method against Staphylococcus aureus and Escherichia coli. The results showed that the yield of resin extract was ranging from 8,44 % to 95,56%. All extracts and essential oil could not inhibit E coli growth but inhibit the S. aureus growth. Resinn-hexane extract from Pinus oocarpa was the most potent as antibacterial activity against S. aureus. All of the samples used had less potential antioxidant activity compared to positive control ascorbic acid.. The potential spot as antioxidant was determined by bioautography and the next step need to separate the active component from the active extract to find the active compounds.

Published
2017-03-08