Visceral Organ Waste as A Substrate for Lipase Production by Bacillus sp. SKII-5

  • Kholilah Nur Hidayah Institut Teknologi Sepuluh Nopember, Indonesia
  • Maharani Pertiwi Koentjoro Shizuoka University, Japan
  • Awik Puji Dyah Nurhayati Institut Teknologi Sepuluh Nopember, Indonesia
  • Endry Nugroho Prasetyo Institut Teknologi Sepuluh Nopember, Indonesia

Abstract

One of solid slaughterhouses waste is visceral organs which contain high lipid content. Besides, this waste has function as medium growth of thermophilic bacteria to produce a wide variety of lipolytic enzymes, such as protease, chitinase, and lipase.Lipase is the most important group of biocatalyst  for biotechnology. The aim of this research is utilize solid slaughterhouses waste of visceral organs (the intestine and rumen) as a substrate to produce lipase from Bacillus sp. SK II-5 and determine the optimum substrate concentration to lipase production.Lipase produce by Bacillus sp. SK II-5 as lipolytic bacteria which confirmed use tween80agar medium. After the results obtained, Bacillus sp. SK II-5 inoculated to starter preparation and acclimatization. Carbon source was provided by fat which isolated from the tissue of bovine intestine and rumen. Substrate with  variation of concentration is 0.11%, 0.21%, 0.31% and 0.42%. The highest lipase activity was determined from substrate 0.11% intestine and 0.21% rumen fat with activity 2858.6 U/ml and 3009.3 U/ml, respectively.  Lipase was isolated by  centrifugation method and toprecipitate by ammonium sulfate, experiment conducted with 15-30%, 30-45%, 45-60%, 60-75% and 75-90%, Results revealed that 60-75% saturation was proved to be effective for maximum specific activity of 1717.3 U/ml and 1642.6 U/ml. Isoelectric point of lipase is pH 5. The molecular weight of lipase which produced by Bacillus sp. SKII-5 is 43,3 kDa by SDS-PAGE

Published
2017-03-08