Biocatalysis of Keratin-Based Waste as A Source Of Soluble Protein

  • Tria Ainur Rosyidah Institut Teknologi Sepuluh Nopember, Indonesia
  • Maharani Pertiwi Koentjoro Shizuoka University , Japan
  • Awik Puji Dyah Nurhayati Institut Teknologi Sepuluh Nopember, Indonesia
  • Endry Nugroho Prasetyo Institut Teknologi Sepuluh Nopember, Indonesia


Keratin waste contains abundant protein that has great potential as a source of nitrogen and amino acids. Keratin-based waste are usually found in modified epidermal tissue of animal, such as hair, fur, wool, and horns. Keratin hydrolysis will produce water soluble protein fractions that are easy to absorb and has high thermal stability. The conversion process of keratin into soluble protein usually using chemical method and steam pressures. But these methods will produce innutritious protein like lantionin and lisinoalanin and will destroy certain amino acids. Alternative and innovative solution to overcome this problem is to convert keratin waste as a soluble protein through keratin hydrolysis using keratinase. This study aims to determine the best sources of keratin which can be converted to soluble protein through hydrolysis. Keratinase enzyme produced by Bacillus sp. SLII-I by fermentation using  feather meal (FM) containing fur medium. Keratinase was isolated using centrifugation method and measured enzyme activity and protein content. Various keratin wastes are converted into soluble proteins enzymatically and the content of soluble protein was observed for each substrate. The results show that Bacillus sp. SLII-I produce the keratinase with 115.56 (mg / sec) / ml enzyme activity. Hydrolysis of keratin waste using keratinase produced the highest soluble protein with 6,60 mg/ml from dog’s hair waste. Therefore from the four wastes, the potential source of soluble protein is the hair of the dog waste.