Isolation, identification, and structure elucidation of saponin compounds from oil-free mahogany seed (Swietenia macrophylla King.)
Saponin compounds have been widely used in the medical sector because of their physiological activities including decreasing blood glucose levels, cholesterol and prevent cancer. This study aims to isolate the saponin compound in the oil-free mahogany seed (Swietenia macrophylla, King) and determine its structure. Mahogany seed oil was isolated by maceration using solvents n-hexane for 3x24 hours. The oil-free residue then macerated using methanol for 3x24 hours. Many as 50 grams of the dried methanol extract was added each 50 mL of distilled water and n-butanol, then shaken, and separated. n-butanol phase was taken and then evaporated to obtain a crude extract of saponin. Further fractionation using vacuum liquid chromatography and radial chromatography with a variety of eluent to obtain the pure compound saponin. The fraction was obtained and analyzed by GC, IR, UV, 1HNMR, and 13CNMR spectrometer. Analysis showed that the compounds found in the extracts were saponin 1,4 -Bis-(3,4,5- trimethoxy-phenyl)-tetrahydro-furo [3,4-c] furan.