Antioxidative Potential of Chitosan and Vitamin C to Lead Intoxication
Internal accumulation of Pb2+ increases reactive oxygen species (ROS) production, which later causes oxidative stress in cells. The purpose of this research is to examine the antioxidative potential of chitosan and vitamin C combination in lead acetate-induced rats. MDA level is used as the main indicator of antioxidant activity.
This experimental laboratory research used 30 male rats. All rats , except five rats for control group, was induced using lead acetate (Pb(CH3COOH)2). 30 rats were divided into six different groups, in which each group was consisted of five rats. Group 1 is control group, meanwhile group 2 is the negative control group treated with lead acetate. Group 3 was treated with 64 mg/kg BW of chitosan/ day. Group 4, 5, and 6 were treated with chitosan and vitamin C combination in dose of 100, 200, and 300 mg/kg BW respectively. Each treatment was given once a day for 40 days. In the 41st day, blood was collected for lead level and MDA analysis.
Result showed that there is a significant difference in blood lead level (sig 0.01) and MDA level (sig 0.00) among all treatment groups. It is shown in dose-dependent manner. As the dose gets higher, the blood lead and MDA level get lower. According to Pearson correlation analysis, there is a positive correlation between blood lead level and MDA, as much as 0.65 (sig 2 tailed) = 0.
Conclusion of this research is combination of chitosan and vitamin C has antioxidative potential to decrease negative effects of lead intoxication.