Effect of Lime Pretreatment on Microstructure of Cassava Stalk Fibers and Growth of Aspergillus niger
(1) Department of Biology, Faculty of Mathematics and Natural Science, Universitas Negeri Semarang
(2) Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada
(3) Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada
(4) Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada
Abstract
Cassava stalk can be converted into sugar-based product by using microorganism. Unfortunately, lignin act as a barrier of optimal bioconversion. Cassava stalk needs pretreatment process for removing this barrier. The effect of lime pretreatment on microstructure of cassava stalk fibers and the growth of Aspergillus niger FNCC 6114 were observed in this research. The cassava stalks were reduced into 0.147- 0.297 mm size and pretreated with 1 % Ca(OH)2. Lime pretreated and unpretreated cassava stalk was used as solid medium for Aspegillus niger FNCC 6114. The effect of pretreatment method on fibers microstructure of cassava stalk was evaluated through SEM micrograph. The growth and metabolism activities of Aspergillus niger FNCC 6114 were monitored through SEM micrograph of media after fermentation. The other parameters examined were changes in glucosamine, reducing sugar levels, and spores’ quantity. Lime pretreatment altered microstructure of cassava stalk fibers. However, cassava stalk without lime pretreatment gave better growth of Aspergillus niger FNCC 6144 based on metabolism activities parameters. Cassava stalks is suitable as media for Aspergillus niger FNCC 6144 through solid state fermentation. For better growth of Aspergillus niger FNCC 6144 fine-sized cassava stalk should not be lime pretreated. The results of this study provide information about the pretreatment of cassava stems which was effective in supporting the growth of Aspergillus niger. Enhancements the utilization of cassava stems by using fungi, for example Aspergillus niger can overcome the accumulation of organic waste that can interfere with environmental sustainability.
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Boumaaza, B., Benkhelifa, M., & Belkhoudja, M. (2015). Effects of Two Salts Compounds on Mycelial Growth , Sporulation , and Spore Germination of Six Isolates of Botrytis cinerea in the Western North of Algeria, 2015.
Guilherme, A. A., Dantas, P. V. F., Santos, E. S., Fernandes, F. A. N., & Macedo, G. R. (2015). Evaluation of Composition, Characterization and Enzymatic Hydrolysis of Pretreated Sugar. Brazilian Journal of Chemical Engineering, 32(01), 23–33. doi:10.1590/0104-6632.20150321s00003146
Jönsson, L. J., & MartÃn, C. (2016). Pretreatment of lignocellulose : Formation of inhibitory by-products and strategies for minimizing their effects. Bioresource Technology, 199, 103–112. doi:10.1016/j.biortech.2015.10.009
Kim, J., Lee, Y. Y., & Kim, T. (2016). A review on alkaline pretreatment technology for bioconversion of lignocellulosic biomass. Bioresource Technology, 199, 42–48. doi:10.1016/j.biortech.2015.08.085
Kumar, P., Barrett, D. M., Delwiche, M. and, & Stroeve, P. (2009). Methods for pretreatment of lignocellulosic biomass for efficient hydrolysis and biofuel production. Industrial & Engineering Chemistry Research, (20 March 2009). doi:10.1021/ie801542g
Millati, R., Niklasson, C., & Taherzadeh, M. J. (2002). Effect of pH , time and temperature of overliming on detoxification of dilute-acid hydrolyzates for fermentation by ... Effect of pH , time and temperature of o v erliming on detoxification of, (January 2014). doi:10.1016/S0032-9592(02)00176-0
Miller, G. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anaytical Chemistry, 31(No.3, March 1959), 426–428.
Nicolás-santiago, S. D. et al, Regalado-gonzález, C., GarcÃa-almendárez, B., & Téllez-jurado, A. (2006). Physiological , morphological , and mannanase production studies on Aspergillus niger uam-gs1 mutants, 9(1). doi:10.2225/vol9-issue1-fulltext-2
Pelaez, H. C., Alfaro, J. R., & Montoya, J. Z. (2013). Simultaneous saccharification and fermentation of cassava stems. Dyna, 80(180), 97–104.
Salihu, A., Abbas, O., Balarabe, A., & Zahangir, S. (2015). Agricultural residues for cellulolytic enzyme production by Aspergillus niger : effects of pretreatment. 3 Biotech, 5, 1101–1106. doi:10.1007/s13205-015-0294-5
Souza, M. M. De, Prietto, L., & Ribeiro, A. C. (2011). Assessment of the antifungal activity of Spirulina platensis phenolic extract against Aspergillus flavus. Avaliação da atividade antifúngica de extrato fenólico de Spirulina platensis contra Aspergillus flavus, 1050–1058.
Sridevi, A. et al, Narasimha, G; Ramanjaneyulu, G., Dillepkumar, K., Reddy, B., & Devi, P. (2015). Saccharification of pretreated sawdust by Aspergillus niger cellulase. 3 Biotech, 5, 883–892. doi:10.1007/s13205-015-0284-7
Widyahapsari, D., Indrati, R., Setyabudi, S., & Sardjono. (2016). Evaluasi perlakuan pendahuluan menggunakan kalsium hidroksida untuk biokonversi jerami padi menjadi L-asam laktat oleh Rhizopus oryzae AT3. AGRITECH, 36(3), 253–260. doi:http://dx.doi.org/10.22146/agritech.16587,ISSN:0216-0455
Yoon, L. W., Ang, T. N., Ngoh, G. C., Seak, A., & Chua, M. (2014). Fungal solid-state fermentation and various methods of enhancement in cellulase production. Science Direct. Biomass and Bioenergy, 67, 319–338. doi:10.1016/j.biombioe.2014.05.013
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