Effects of BAP and Lighting Duration on Banana (Musa paradisiaca cv. Raja Bulu) Micropropagation
(1) Master Program of Agricultural Biotechnology, Postgraduate Program, Universitas Jenderal Soedirman
(2) Master Program of Agricultural Biotechnology, Postgraduate Program, Universitas Jenderal Soedirman Faculty of Agriculture, Universitas Jenderal Soedirman
(3) Faculty of Biology, Universitas Jenderal Soedirman
Abstract
This study aims to determine the best concentration of 6-Benzilaminopurine (BAP) and lighting duration for Raja Bulu banana cultivar micropropagation. The explant was taken from Pagubugan Village, Cilacap Regency, Central Java. The experiment was arranged as a Randomized Completely Block Design (RCBD) with two factors. The first factor was BAP, with concentration used were 0; 2.5; 5; 7.5 mg/L and the addition of Indole-3-acetic acid (IAA) 0.5 mg/L for all treatment. The second factor was lighting treatment. All explants were put in dark incubation for two weeks followed by light duration treatment: 12 hours of light/day and 16 hours of light/day for the next two weeks. The addition of BAP increased the number of shoots, but did not affect time for shoot initiation, shoot height, number of leaves and number of roots. The best BAP concentration was 2.5 mg/L with an average number of 3.2 shoots/explant. Scalps were formed in the treatment of BAP 5 mg/L and 7 mg/L after four weeks of incubation and has the potential to become new shoots. Nevertheless, the lighting duration did not affect all observed variables.
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Abdel-Aal, A, M. Aboul-Nasr, S. EL-Massry, and W. Pisam. 2016. “Effect of Magnesium Sulphate and Light Duration on Multiplication Rate of Banana (Musa Sp.) in Vitro Culture.” Journal of Plant Production 7(2): 105–9. https://jpp.journals.ekb.eg /article_44269.html.
Annisa, Raden Roro Rifka, Arkan Setiaji, and Aries Bagus Sasongko. 2020. “In Vitro Shoot Induction of Musa Acuminata Cv. Mas Kirana.” Quagga: Jurnal Pendidikan dan Biologi 13(1): 34. https://journal.uniku.ac.id/index.php/quagga/article/view/3431.
Bhaya, Mohameed Hani Majeed, and Siham Abd Al-RazzaqSalim. 2019. “Impacts of Plant Growth Regulators and Light Quality on Banana (Musa Spp ) Micropropagation.” Plant Archives 19(1): 1379–85.
Deo, Bandita, and Bikram Pradhan. 2017. “Effects of Plant Growth Hormones on Shoot Proliferation of Musa Paradisiaca Cv. Bantal.” International Journal of Plant Sciences 12(2): 135–38.
Dobisova, Tereza et al. 2017. “Light Controls Cytokinin Signaling via Transcriptional Regulation of Constitutively Active Sensor Histidine Kinase CKI1.” Plant Physiology 174(1): 387–404. http://www.plantphysiol.org/lookup/doi/10.1104/pp.16.01964.
Elma, Tiara et al. 2017. “Multiplikasi Tunas Mikro Pisang (Musa Paradisiaca l.) ‘Raja Bulu’ Secara in Vitro Pada Berbagai Jenis Dan Konsentrasi Sitokinin.” Kultivasi 16(3): 418–24.
Falcomer, Ana Luisa et al. 2019. “Health Benefits of Green Banana Consumption: A Systematic Review.” Nutrients 11(6): 1222. https:// www.mdpi.com/2072-6643/11/6/1222.
Hapsoro, D., D. Saputra, and Yusnita. 2017. “Pengaruh Konsentrasi Benziladenin Dan Sukrosa Terhadap Multiplikasi Tunas Pisang Raja Bulu (AAB) In Vitro.” In Mendorong Kedaulatan Pangan Melalui Pemanfaatan Sumber Daya Unggul Lokal, Balunijuk: Fakultas Pertanian, Perikanan, dan Biologi Universitas Bangka Belitung.
Kumar, K P Sampath, Debjit Bhowmik, S Duraivel, and M Umadevi. 2012. “Traditional and Medicinal Uses of Banana.” Journal of Pharmacognosy and Phytochemistry 1(3): 51–63.
Lim, T. K. 2012. “Musa Acuminata × Balbisiana (AAB Group) ‘Pisang Raja.’” In Edible Medicinal And Non Medicinal Plants, Dordrecht: Springer Netherlands, 551–53. http://link.springer.com/ 10.1007/978-94-007-2534-8_72.
Mohammed, Binish et al. 2018. “Converging Light, Energy and Hormonal Signaling Control Meristem Activity, Leaf Initiation, and Growth.” Plant Physiology 176(2): 1365–81. http://www. plantphysiol.org/lookup/doi/10.1104/pp.17.01730.
Napier, R.M. 2017. “Auxins.” In Encyclopedia of Applied Plant Sciences, Elsevier, 367–77. https://linkinghub.elsevier.com/retrieve/pii/B978012394807600099X.
Peer, Wendy Ann, Joshua J. Blakeslee, Haibing Yang, and Angus S. Murphy. 2011. “Seven Things We Think We Know about Auxin Transport.” Molecular Plant 4(3): 487–504. http://dx.doi.org/10.1093/mp/ssr034.
Pereira, Gustavo Alves et al. 2018. “Concentrations of 6-Benzylaminopurine (BAP) in Micro propagation of Banana ‘Farta Velhaco’ (AAB).” Comunicata Scientiae 9(1): 58–63. https://www. comunicatascientiae.com.br/comunicata/article/view/2034.
Ratnasari., Bella Dian, Erni Suminar, Anne Nuraini, and Ade Ismail. 2016. “Pengujian Efektivitas Berbagai Jenis Dan Konsentrasi Sitokinin Terhadap Multiplikasi Tunas Mikro Pisang (Musa Paradisiaca L.) Secara In Vitro.” Kultivasi 15(2). http://jurnal.unpad.ac.id/kultivasi/article/view/11870.
Sari, Widya et al. 2018. “Keanekaragaman Dan Patogenisitas Fusarium Spp. Asal Beberapa Kultivar Pisang.” Jurnal Fitopatologi Indonesia 13(6): 216. https://jagb.journal.ipb.ac.id/index. php/jfiti/article/view/21884.
Shahnawaz, Ahmed et al. 2014. “In Vitro Multiplication of Banana (Musa Sp.) Cv. Grand Naine.” African Journal of Biotechnology 13(27): 2696–2703. http://academicjournals.org/journal/AJB/article-abstract/106134B45706.
Singh, H P et al. 2011. Micropropagation for Production of Quality Banana Planting Material in Asia-Pacific Consortium on Agricultural Biotechnology (APCoAB). APAARI.
Sipen, Philip, and Michael R Davey. 2012. “Effects of N6-Benzylaminopurine and Indole Acetic Acid on in Vitro Shoot Multiplication, Nodule-like Meristem Proliferation and Plant Regeneration of Malaysian Bananas (Musa Spp.).” Tropical Life Sciences Research 23(2): 67–80.
Soesanto, Loekas, Endang Mugiastuti, and Fajarudin Ahmad. 2013. “Diagnosis Lima Penyakit Utama Karena Jamur Pada 100 Kultivar Bibit Pisang.” Jurnal Hama dan Penyakit Tumbuhan Tropika 12(1): 36–45.
Srivastava, Vartika, A. K. Singh, and S. P. Singh. 2012. “Optimization of BAP and IAA Concentration on Shoot Induction, Proliferation and Rooting in Shoot-Tip Culture of Banana Cv. Dwarf Cavendish.” Indian Journal of Plant Physiology 17(2): 179–84.
Street, Ian H. et al. 2016. “Cytokinin Acts through the Auxin Influx Carrier AUX1 to Regulate Cell Elongation in the Root.” Development 143(21): 3982–93. http://dev.biologists.org/lookup/doi/10.1242/dev.132035.
Sukmadjaja, Deden, Ragapadmi Purnamaningsih, and Tri P. Priyatno. 2016. “Seleksi in Vitro Dan Pengujian Mutan Tanaman Pisang Ambon Kuning Untuk Ketahanan Terhadap Penyakit Layu Fusarium.” Jurnal AgroBiogen 9(2): 66.
Suseno, Nilo. 2017. “Micropropagation of Banana Plant (Musa Paradisiaca) Cv . Raja Bulu through Tissue Culture for Diversification of Food and Feed.” In The 7th International Seminar on Tropical Animal Production Contribution of Livestock Production on Food Sovereignty in Tropical Countries, Yogyakarta, 795–98.
Triharyanto, E, R. B. Arniputri, E. S. Muliawati, and E. Trisnawati. 2018. “Studies Concentration of IAA and BAP on Multiplication of Raja Bulu Banana in Vitro and the Acclimatization.” Agrotechnology Research Journal 2(1): 1–5.
Trivedi, Ankita, and R S Sengar. 2017. “Effect of Various Light-Emittimg Diodes on Growth and Photosynthetic Pigments of Banana ( Musa Acuminata ) Cv . Grande Naine in Vitro Plantlets.” International Journal of Chemical Studies 5(5): 1819–21.
Van-Gelderen, Kasper, Chiakai Kang, and Ronald Pierik. 2018. “Light Signaling, Root Development, and Plasticity.” Plant Physiology 176(2): 1049–60. http://www.plantphysiol.org/ lookup/doi/10.1104/pp.17.01079.
Waidmann, Sascha et al. 2019. “Cytokinin Functions as an Asymmetric and Anti-Gravitropic Signal in Lateral Roots.” Nature Communications 10(1): 3540. http://www.nature.com/articles/s41467-019-11483-4.
Yusnita, Yusnita, Ekawati Danial, and Dwi Hapsoro. 2015. “In Vitro Shoot Regeneration of Indonesian Bananas (Musa Spp.) Cv. Ambon Kuning and Raja Bulu, Plantlet Acclimatizationand Field Performance.” AGRIVITA 37(1): 51–58. https:// agrivita.ub.ac.id/index.php/agrivita/article/view/438.
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