Potential of Calliandra calothyrsus Leaf Extract to Maintain Estrogen Concentration and Uterine Thickness in Rats

Iriani Setyawati(1), Ni Nyoman Wirasiti(2), Luh Putu Eswaryanti Kusuma Yuni(3),

DOI: https://doi.org/10.15294/biosaintifika.v13i2.31063


(1) Program Study of Biology, Faculty of Mathematics and Natural Sciences, Universitas Udayana, Indonesia
(2) Program Study of Biology, Faculty of Mathematics and Natural Sciences, Universitas Udayana, Indonesia
(3) Program Study of Biology, Faculty of Mathematics and Natural Sciences, Universitas Udayana, Indonesia

Abstract

Calliandra calothyrsus Meissn. leaf extract is potential as phytoestrogens. It influenced male mice reproduction, rat estrous cycle, and ovarian histology in previous study. This research aimed to prove the C. calothyrsus leaf extract potential as phytoestrogen source and the effect on endometrial thickness where the embryo implantation take place in early pregnancy. This study used a Completely Randomized Design used 54 days old female rats (Rattus norvegicus). Rats were divided into K as control group (treated with 0.5% Na-CMC as placebo) and P1, P2, and P3 as three groups with C. calothyrsus leaf extract administration with doses of 17.5; 35; and 70 mg/ kg bw respectively. Treatments were given 1 ml/rat/day orally for 20 days. At day 21st, animals were euthanized to collect blood samples for estrogen hormone analysis. After the dissection, all uterus were collected and weighed. Histological preparation was done with paraffin method and Hematoxylin-Eosin staining. The effective dose was 70 mg/ kg bw that did not decrease the weight of the uterus and the body. This dose even maintained the normal diameter and thickness of uterine walls (endometrium, myometrium, and perimetrium layers) like control rats. The extract in this study could increase estrogen concentration in female rats. This research novelty is that C. calothyrsus leaf extract (70 mg/ kg bw) can be used as an alternative herbal suplement to maintain uterine wall thickness and estrogen concentration in productive women. With further clinical research, this extract is a good candidate as potential estrogen source to overcome women infertility or pregnancy difficulties due to problem of endometrial thickness and lack of endogenous estrogen.

Keywords

Calliandra calothyrsus, estrogen, endometrium, myometrium, perimetrium

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References

Abd-Elkareem. (2017). Morphological, histological and immunohistochemical study of the rabbit uterus during pseudopregnancy. Journal of Cytology & Histology, 8, 443. https://doi.org/10.4172/2157-7099.1000443

Al-Sabbagh, M., Lam, E. W., & Brosens, J. J. (2012). Mechanisms of endometrial progesterone resistance. Molecular and Cellular Endocrinology, 358(2), 208-215. https://doi.org/10.1016/j.mce.2011.10.035

Ameer, M.A., Fagan, S. E., & Sosa-Stanley, J. N. (2021). Anatomy, abdomen and pelvis, uterus. StatPearls Publishing. https://www.ncbi.nlm.nih.gov/books/NBK470297/

Barrett, K. E., Barman, S. M., Brooks, H. L. & Yuan, J. (2019). Ganong’s Review of Medical Physiology. 26th Edition. Mc.Graw Hill Book.

Chung, D., Gao, F., Jegga, A. G., & Das, S. K. (2015). Estrogen mediated epithelial proliferation in the uterus is directed by stromal Fgf10 and Bmp8a. Molecular and Cellular Endocrinology, 400, 48-60. https://doi.org/10.1016/j.mce.2014.11.002

Constantine, G., Millheiser, L. S., Kaunitz, A. M., Parish, S. J., Graham, S., Bernick, B., & Mirkin, S. (2019). Early onset of action with a 17β-estradiol, softgel, vaginal insert for treating vulvar and vaginal atrophy and moderate to severe dyspareunia. Menopause, 26(11), 1259-1264. https://doi.org/10.1097/GME.0000000000001394

Fitmawati, Sofiyanti, N., Roza, R. M., Isnaini, Irawan, Y. R., Winata, D. R., & Dewi, A. P. K. (2017). Antioxidant activity of dominant plants species in obat pahit from lingga malay ethnic in riau archipelago. Biosaintifika: Journal of Biology & Biology Education, 9(2), 325-331. https://doi.org/10.15294/biosaintifika.v9i2.9808

Gultom, Y. N. (2001). Efektivitas penyuntikan PMSG sebelum perkawinan induk pada bobot organ dan ukuran tubuh anak tikus putih usia lepas sapih [Unpublished thesis]. Institut Pertanian Bogor.

Hall, J. (2015). Guyton and Hall Textbook of Medical Physiology, 13th Edition, Saunders Publisher.

Hamilton, K. J., Hewitt, S. C., Arao, Y., & Korach, K. S. (2017). Estrogen hormone biology. Current Topics in Developmental Biology, 125, 109-146. https://doi.org/10.1016/bs.ctdb.2016.12.005

Holesh, J. E., Bass, A. N., & Lord, M. (2021). Physiology, ovulation. StatPearls Publishing. https://www.ncbi.nlm.nih.gov/books/NBK441996/

Johnson, M. H. (2018). Essential Reproduction, 8th Edition. Wiley-Blackwell.

Kelleher, A. M., Burns, G. W., Behura, S., Wu, G., & Spencer, T. E. (2016). Uterine glands impact uterine receptivity, luminal fluid homeostasis and blastocyst implantation. Scientific Reports, 6, 38078. https://doi.org/10.1038/srep38078

Kota, S. K., Gayatri, K., Jammula, S., Kota, S. K., Krishna, S. V. S., Meher, L. K. & Modi, K. D. (2013). Endocrinology of parturition. Indian Journal of Endocrinology and Metabolism, 17(1), 50-59. https://doi.org/10.4103/2230-8210.107841

Kumar, P., & Sait, S. F. (2011). Luteinizing hormone and its dilemma in ovulation induction. Journal of Human Reproductive Sciences, 4(1), 2-7. https://doi.org/10.4103/0974-1208.82351

Lephart, E. D. (2015). Modulation of aromatase by phytoestrogens. Enzyme Research, Article ID 594656. https://doi.org/10.1155/2015/594656

Mardika, K., Setyawati, I., & Darmadi, A. A. K. (2018). Panjang siklus estrus dan struktur histologi ovarium tikus putih setelah pemberian ekstrak etanol daun kaliandra merah. Jurnal Veteriner, 19(3), 342-350.

Marquardt, R. M., Kim, T. H., Shin, J. H., & Jeong, J. W. (2019). Progesterone and estrogen signaling in the endometrium: what goes wrong in endometriosis?. International Journal of Molecular Sciences, 20(15), 3822. https://doi.org/10.3390/ijms20153822

Narulita, E., Prihatin, J., & Dewi, R. S. (2016). Pemanfaatan hasil induksi hormon estrogen terhadap kadar estradiol dan histologi uterus mencit (Mus musculus) sebagai buku suplemen sistem reproduksi di SMA, Jurnal Bioedukatika, 4(2), 1-7.

Onyeama, H. P., Ibekwe, H. A., Ofemile, P. Y., Peter, A., Ahmed, M. S., & Nwagbo, P. O. (2012). Screening and acute toxicity studies of calliandra portoricensis (eri agbo in igbo) used in the treatment of snake bite in south eastern nigeria. Vom Journal of Veterinary Science 9, 17-24.

Paterni, I., Granchi, C., Katzenellenbogen, J. A., & Minutolo, F. (2014). Estrogen receptors alpha (ERα) and beta (ERβ): subtype-selective ligands and clinical potential. Steroids, 90, 13-29. https://doi.org/10.1016/j.steroids.2014.06.012

Resende, F. A., de-Oliveira, A. P. S., de-Camargo, M. S., Vilegas, W., & Varanda, E. A. (2013). Evaluation of estrogenic potential of flavonoids using a recombinant yeast strain and MCF7/BUS cell proliferation assay, PLoS One, 8(10), e74881. https://doi.org/10.1371/journal.pone.0074881

Rietjens, I. M., Sotoca, A. M., Vervoort, J., & Louisse, J. (2013). Mechanisms underlying the dualistic mode of action of major soy isoflavones in relation to cell proliferation and cancer risks. Molecular Nutrition & Food Research, 57(1), 100-113. https://doi.org/10.1002/mnfr.201200439

Roberts, H, & Lethaby, A. (2014). Phytoestrogens for menopausal vasomotor symptoms: a cochrane review summary, Maturitas, 78(2), 79-81. https://doi.org/10.1016/j.maturitas.2014.04.004

Schweikart, K. M., Eldridge, S. R., Safgren, S. L., Parman, T., Reid, J. M., Ames, M. M., Goetz, M. P., & Davis, M. A. (2014). Comparative uterotrophic effects of endoxifen and tamoxifen in ovariectomized sprague-dawley rats. Toxicologic Pathology, 42(8), 1188-1196. https://doi.org/10.1177/0192623314525688

Setyawati, I, Wijayanti, N. P. A. D., & Wiratmini, N. I. (2019). Phytochemical content, extract standardization and antioxidant activity of Calliandra calothyrsus Meissn leaf, a potential phytoestrogen source, IOP Conference Series: Earth and Environmental Science, 347, Article 012075.

Thomas, C., & Gustafsson, J. Å. (2011). The different roles of ER subtypes in cancer biology and therapy, Nature Reviews Cancer, 11(8), 597-608. https://doi.org/10.1038/nrc3093

Thiyagarajan, D. K., Basit, H., & Jeanmonod, R. Physiology, menstrual cycle. StatPearls Publishing. https://www.ncbi.nlm.nih.gov/books/NBK500020/

Widhayanthi, T. A., Pangkahila, W., & Pinatih, G. N. I. (2017). Pemberian suplemen swissoats A3® meningkatkan kadar hormon estrogen pada tikus (Rattus norvegicus) betina menopause galur wistar, Jurnal Biomedik, 9(2), 75-81.

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