Respon Kecambah Padi (Oryza sativa L.) Asal Bengkalis, Riau Terhadap Cekaman Garam
(1) Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Riau, Kampus Bina Widya, Jl. HR Soebrantas, Panam, Pekanbaru 28293, Riau, Indonesia
(2) Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Riau, Kampus Bina Widya, Jl. HR Soebrantas, Panam, Pekanbaru 28293, Riau, Indonesia
(3) Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Riau, Kampus Bina Widya, Jl. HR Soebrantas, Panam, Pekanbaru 28293, Riau, Indonesia
Abstract
Varietas padi yang tahan terhadap cekaman garam sangat diperlukan untuk mengatasi masalah cekaman garam di wilayah pesisir pantai. Penelitian ini bertujuan menganalisis respon pertumbuhan akar dan tajuk serta pertambahan biomassa akar dan tajuk dari enam varietas padi pada fase kecambah. Varietas padi lokal yang digunakan berasal dari Kecamatan
Bantan, Kabupaten Bengkalis, yaitu Amat Candu, Sadani, Solok, dan Yamin. Dua varietas pembanding yang digunakan adalah IR64 yang tidak tahan dan Indragiri yang tahan terhadap cekaman garam. Penelitian dilakukan menggunakan Rancangan Acak Kelompok dengan dua faktor dan tiga kali ulangan. Faktor pertama adalah konsentrasi NaCl, yaitu 0 mM, 15 mM, 30 mM, dan 45 mM. Faktor kedua adalah varietas padi. Hasil penelitian menunjukkan bahwa di antara empat varietas lokal yang diuji, varietas Amat Candu memberikan respon pertumbuhan dan biomassa akar paling baik sehingga dapat digolongkan ke dalam varietas padi yang moderat terhadap cekaman garam; sedangkan tiga varietas padi lainnya, yakni Solok, Sadani, dan Yamin tergolong tidak tahan cekaman garam. Pertumbuhan akar kecambah padi pada perlakuan cekaman garam 30 mM merupakan karakter yang dapat membedakan ketahanan terhadap cekaman garam. Oleh karena itu pertumbuhan akar kecambah pada cekaman garam 30mM disarankan untuk digunakan dalam penapisan varietas padi untuk ketahanan terhadap cekaman garam.
Salt tolerant rice varieties is needed to overcome the problem of salt stress on coastal areas. This study aimed to analyze the roots and canopy growth of six rice varieties at germination stage. Four local rice varieties used derived from District of Bantan, Bengkalis, i.e. Amat Candu, Sadani, Solok, and Yamin. Two control varieties, IR64 and Indragiri, were used as salt sensitive and salt tolerance, respectively. The study was conducted using a randomized block design with two factors and three replications. The first factor was concentration of NaCl, i.e. 0 mM, 15 mM, 30 mM, and 45 mM. The second factor was the rice variety. The results showed that among four local varieties tested, Amat Candu varieties had best root growth and biomass; therefore it can be classified into a moderate tolerance to salt stress; while three others, i.e. Solok, Sadani, and Yamin classified as salt sensitive varieties. Seedling root growth at 30 mM salt stress treatment could differentiate the salt resistance salt. Hence, the growth of seedling root on salt stress 30 mM is recommended for use in rice varieties screening for resistance to salt stress.
Keywords
Full Text:
PDFReferences
Abbas, M. K., Ali, A. S., Hasan, H. H., & Ghal, R. H. (2013). Salt tolerance study of six cultivars of rice (Oryza sativa L.) during germination and early seedling growth. Journal of Agricultural Science, 5(1). doi:10.5539/jas.v5n1p250.
Anbumalarmath, J., & Mehta, P. (2013). Effect of Salt Stress on Germination of indica Rice Varieties. EJBS 6(1). www.ejarr.com/Volumes/Vol6/EJBS_6_01.pdf.
Batlang, U., Baisakh, N., Ambavaram, M. M., & Pereira, A. (2013). Phenotypic and physiological evaluation for drought and salinity stress responses in rice. Methods Mol Biol. 956, 209-25. doi:10.1007/978-1-62703-194-3_15.
Blumwald, E., Aharon, G. S., & Apse, M. P. (2000). Sodium transport in plant cells. Biochimica et Biophysica Acta, 1465, 140-151.
Fitmawati, Herman, & Sofiyanti, N. (2012). Keanekaragaman jenis dan pola distribusi geografis mangga (Mangifera) menggunakan spatial modelling yang mengintegrasikan data ekologi dan geografi dalam analisisnya. Laporan Penelitian Fundamental Tahun 1. Pekanbaru: Universitas Riau.
Ibraheem, O., Dealtry, G., Roux, S., & Bradley, G. (2011). The effect of drought and salinity on the expressional levels of sucrose transporters in rice (Oryza sativa Nipponbare) cultivar plants. POJ, 4(2), 68-74.
Juma, N. G. 1999). The Pedosphere and Its Dynamics. Vol. 1 Introduction to Soil Science and Soil Resources. Salman Production Inc.
Nam, M. H., Huh, S. M., Kim, K. M., Park, W. J., Seo, JB, Cho K, Kim DY, Kim BG, Yoon IS. (2012). Comparative proteomic analysis of early salt stress-responsive proteins in roots of SnRK2 transgenic rice. Proteome Science, 10(1), 25-44.
Mandal, A. B., Basu AK, Roy B, Sheeja TE, & Roy T. (2004). Genetic management for increased tolerance to aluminium and iron toxicities in rice – A review. Indian J Biotechnol, 3, 359-368.
Miftahudin, Scoles, G. J., & Gustafson, J. P. (2002). AFLP markers tightly linked to the aluminum-tolerance gene Alt3 in rye (Secale cereale L.). Theor Appl Genet, 104, 626-631.
Munns, R. (2002). Comparative physiology of salt and water stress. Plant, Cell and Environment, 25, 239–250.
Omokawa, H., & Aomuma, S. (2002). Amelioration of the salt-stressed root growth of rice and normalization of the Na+ distribution between the shoot and root by (S)-a-Methylbenzyl-2-‰uoro-4-methylphenylurea. Biosci. Biotechnol. Biochem, 66(2), 336–343.
Pattanagul, W., & Thitisaksakul, M. (2008). Effect of salinity stress on growth and carbohydrate metabolism in three rice (Oryza sativa L.) cultivars differing in salinity tolerance. Indian Journal of Experimental Biology, 46(10): 736-742.
Romero-Aranda R, Syvertsen J P. (1996). The influence of foliar applied urea nitrogen and saline solutions on net gas exchange of Citrus leaves. J Amer Soc Hort Sci 121:501-506.
Roslim DI, Miftahudin, Suharsono U, Aswidinnoor H, Hartana A. (2010). Karakter root re-growth sebagai parameter toleransi aluminium pada tanaman padi. Jurnal Natur Indonesia 13(1):82-88.
Silitonga TS. (2004. Pengelolaan dan Pemanfaatan Plasma Nutfah Padi di Indonesia. Buletin Plasma Nutfah 10(2):56-71.
Siringam K, Juntawong N, Cha-um S, Kirdmanee C. (2011). Salt stress induced ion accumulation, ion homeostasis, membrane injury and sugar contents in salt-sensitive rice (Oryza sativa L. spp. indica) roots under isoosmotic conditions. African Journal of Biotechnology 10(8): 1340-1346.
Slavich P, McLeod M, Moore N, Iskandar T, Rachman A. (2006). Mengatasi pengaruh salinitas terhadap pertumbuhan tanaman di lahan yang terkena dampak tsunami di provinsi Nanggroe Aceh Darussalam, Indonesia. Nanggro Aceh Darussalam: BPTP NAD, Balai Penelitian Tanah Indonesia.
Subbarao GV. (2002). Physiological Mechanisms Relevant to Genetic Improvement of Salinity Tolerance in Crop Plants. In: Mohammad Pessarakli. Handbook of Plant and Crop Physiology (second edition). New York: Marcel Dekker, Inc.
Wang H, Meishan Zhang2, Rui Guo3, Decheng Shi1, Bao Liu1, Xiuyun Lin4 and Chunwu Yang. (2012). Effects of salt stress on ion balance and nitrogen metabolism of old and young leaves in rice. (Oryza sativa L.). BMC Plant Biology 12:194.
Wangxia Wang, Vinocur B, Altman A. (2003). Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta 218: 1–14. DOI 10.1007/s00425-003-1105-5.
Yamamoto A, Sawada H, Shim IS, Usui K, Fujihara S. (2011). Effect of salt stress on physiological response and leaf polyamine content in NERICA rice seedlings. Plant Soil Environ 57(12): 571–576.
Zhen-hua Z, Qiang L, Hai-xing S, Xiang-min R, Ismail AM. (2012). Responses of different rice (Oryza sativa L.) genotypes to salt stress and relation to carbohydrate metabolism and chlorophyll content. African Journal of Agricultural Research 7(1): 19-27.
Refbacks
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution 4.0 International License.