In Vitro Antiinflammatory Activity of Bajakah (Spatholobus littoralis) Stem Extract
(1) Tanjungpura University
(2) Tanjungpura University
Abstract
The plant of Bajakah tampala (Spatholobus littoralis Hassk) has been utilized in traditional medication. Previous studies have proven the existence of in vivo anti-inflammatory activities of Bajakah plant (S. littoralis) in lowering the degree of carrageenan-induced paw oedema in mice. This study aims to determine the anti-inflammatory mechanism of S. littoralis extract in vitro through an approach of enzyme inhibition involved in the inflammatory reaction. The concentration of ethanol extract of Bajakah used was 0.1; 0.2; 0.4; 0.8; 1.6 mg/ml. The parameters measured were lipoxygenase enzyme inhibition, protein denaturation inhibition, protease enzyme inhibition, as well as plasma membrane stabilization. The results of the study showed the potential of the ethanol extract of Bajakah stems in inhibiting the inflammatory process viewed from the ability to inhibit inflammation-related enzymes. S. littoralis extract concentration of 1.6 mg/ml showed the best inhibition of the protein denaturation process (75.9%), the inhibition of trypsin protease enzyme (26.1%) and the stability of erythrocyte membrane (93.7%). However, the extracts of S. littoralis did not provide inhibition for the lipoxygenase enzyme in the range of 0.2-3.8%. This study proves the role of S. littoralis extract in the anti-inflammatory mechanism. It has the potential to be developed into standardized herbs.
Keywords
Full Text:
PDFReferences
Adnan, A.T., Armin, F., Sudji, I., Novida, M.D., Roesma, D.I., Ali, H.A., Fauzana, A. (2019). In Vitro Anti-Inflammatory Activity Test of Tinocrisposide and Freeze-Dried Aqueous Extract of Tinospora crispa Stems on Human Red Blood Cell by Increasing Membrane Stability Experiment. Asian Journal of Pharmaceutical and Clinical Research, 12(5), 125-129 DOI: http://dx.doi.org/10.22159/ajpcr.2019.v12i5.30690
Anosike, C.A., Obidoa, O., Ezeanyika, L.U.S. (2012). Membrane stabilization as a mechanism of the anti-inflammatory activity of methanol extract of garden egg (Solanum aethiopicum). Daru Journal of Pharmaceutical Sciences, 20(1), 76 http://www.darujps.com/content/20/1/76.
Bonam, S.R., Wang, F., Muller, S. (2019). Lysosomes as a therapeutic target, Nature Reviews, 18, 923-948.
Elisha, I.L., Dzoyem, J., McGaw, L.J., Botha, F.S., Eloff, J.N. (2016). The anti-arthritic, anti-inflammatory, antioxidant activity and relationship with total phenolics and total flavonoids of nine South African plants used traditionally to threat arthritis. BMC Complement Alternative Medicine, 16(1), 307, doi: 10.1186/s12906-016-1301-z.
Fitmawati, Roza,R.M., Sofiyanti, N., Isnaini, Fitri, F.L., Paramita, D., Kusumo, A.P. (2017). Immunomodulatory Effectiveness of Aqueous Obat Pahit Extract of Lingga Malay Ethnic on White Rats (Rattus novergicus). Biosaintifika: Journal of Biology and Biology Education, 9(3), 430-436 DOI: 10.15294/biosaintifika.v9i3.10496
Gambhire, M., Juvekar, A. Wankhede, S. (2009). Evaluation of the anti-inflammatory activity of methanol extract of Barleria cristata leaves by in vivo and in vitro methods. Int. J. Pharmacol 7, 1–6
Gunathilake, K.D.P.P., Ranaweera, K.K.D.S., Rupasinghe, H.P.V. (2018). Influence of boiling, steaming and frying of selected leafy vegetables on the in vitro anti-inflammation associated biological activities, Plants 7, 22.
Harirforoosh, S., Asghar, W., Jamali, F. (2013). Adverse Effects of Nonsteroidal Antiinflammatory Drugs: An Update of Gastrointestinal, Cardiovascular and Renal Complications. Journal of Pharmacy and Pharmaceutical Sciences, 16(5), 821-847, DOI 10.18433/J3VW2F
Hasan, A.U.H. (2019). Evaluation of in vitro and in vivo therapeutic efficacy of Ribes alpestre Decne in Rheumatoid arthritis. Brazilian Journal of Pharmaceutical Sciences, 55, e17832 1-8 http://dx.doi.org/10.1590/s2175-97902019000217832
Huang, Y., Chen, L., Feng., L., Guo, F. (2013). Characterization of Total Phenolic Constituents from the Stems of Spatholobus suberectus Using LC-DAD-MSn and Their Inhibitory Effect on Human Neutrophil Elastase Activity. Molecules, 18, 7549-7556.
Inami, K., Asada, Y., Harada, T., Okayama, Y., Usui, N., Mochizuki, M. (2019). Antimutagenic components in Spatholobus suberectus Dunn against N-methyl-Nnitrosourea. Genes and Environment Journal, 41, 22-27.
Li, Q., Wei, Q., Yuan, E., Yang, J., Ning, Z. (2013). Interaction Between Four Flavonoids and Trypsin: Effect on The Characteristics of Trypsin and Antioxidant Activity of Flavonoids. International Journal of Food Sciences and Technology, 49(4), 1063-1069 DOI https://doi.org/10.1111/ijfs.12401.
Ninkaew, S. & Chantaranothai. (2014). The Genus Spatholobus Hassk. (Leguminosae-Papilionoideae) in Thailand. Tropical Natural History, 14(2), 87-99.
Novanty, V., Pangkahila, W., Dewi, N.N.A. (2021). Administration of Ethanol Extract of Bajakah Tampala (Spatholobus littoralis Hassk.) Stem Decreased Reactive Oxygen Species, Visceral Fat and Body Weight of Obese Rats. Neurologico Spinale Medico Chirurgico, 4(1), 32-36 doi: 10.18433/J3VW2F.
Okoli, C.O., Akah, P.A., Onuoha, N.J., Okoye, T.C., Nwoye, A.C., Nworu, C.S. (2008). Acanthus montanus: An experimental evaluation of the antimicrobial, anti-inflammatory and immunological properties of a traditional remedy for furuncles. BMC Complement. Altern. Med. 8, 27.
Patel, S.S. & Zaveri, M.N. (2014). Trypsin and Protein Denaturation Inhibitory Activity of Different Fractionation and Isolated Compound of Leaf and Root of Justicia gendarussa. International Journal of Pharmaceutical Sciences and Research, 5(12), 5564-5571 doi 10.13040/IJPSR.0975-8232.5(12).5564-71
Paul, S., Modak, D., Chattaraj, S., Nandi, D., Sarkar, A., Roy, J., Chaudhuri, T.K., Bhattacharjee, S. (2021). Aloe vera gel homogenate shows anti-inflammatory activity through lysosomal membrane stabilization and downregulation of TNF-α and Cox-2 gene expressions in inflammatory arthritic animals. Future Journal of Pharmaceutical Sciences, 7(12), 1-8 https://doi.org/10.1186/s43094-020-00163-6
Pizzino, G., Irrera, N., Cucinotta, M., Pallio, G., Manino, F., Arcoraci, V., Squadrito, F., Altavilla, D., Bitto, A. (2017). Oxidative Stress: Harms and Benefits for Human Health. Oxidative Medicine and Cellular Longevity 1-13, DOI: 10.1155/2017/8416763
Precupas, A., Sandu R., Popa VT. (2016). Quercetin Influence on Thermal Denaturation of Bovine Serum Albumin. J. Phys. Chem. 120(35), 9362–9375 https://doi.org/10.1021/acs.jpcb.6b06214
Rissyelly, Aziz, S., Sangande, F., Mahatya, A.W.M., Budipramana, K., Elfahmi, Sukrasno. (2022). The Inhibition of 15-Lipoxygenase by Blechnum orientale Leaves and its Glycoside-flavonoid Isolates: In Vitro and In Silico Studies. Hayati Journal of Biosciences, 29(3), 353-359 DOI:10.4308/hjb.29.3.353-359.
Rousdy, D.W., Wardoyo, E.R.P., Ifadatin, S. (2022). Anti-inflammatory Activity of Bajakah Stem (Spatholobus littoralis Hassk.) Ethanolic Extract in Carrageenan-Induced Paw Edema Mice. Jurnal Biodjati, 7(1), 66-74.
Saputera, M.M.A., Marpaung, T.W.A., Ayuchecaria, N. (2019). Konsentrasi Hambat Minimum (KHM) Kadar Ekstrak Etanol Batang Bajakah Tampala (Spatholobus littoralis Hassk) Terhadap Bakteri Escherichia coli Melalui Metode Sumuran. Jurnal Ilmiah Manuntung, 5(2), 167-173.
Sakat, S., Juvekar, A.R., Gambhire, M.N. (2010). In vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata Linn. Int. J. Pharm. Pharm. Sci. 2, 146–155.
Shao, Z. (2017). Spatholobus suberectus Stem Extract Improves The Protective Effect Of Heparin On Cerulein–Induced Pancreatitis. Afr J Tradit Complement Altern Med., 14 (3), 187-193.
Sheshachalam, A., Srivastava, N., Mitchell, T., Lacy, P., Eitzen, G. (2014). Granule Protein Processing and Regulated Secretion in Neutrophils. Frontiers in Immunology, 5, 1-11.
Shinde, U.A., Phadke, A.S., Nari, A.M., Mungantiwar, A.A., Dikshit, V.J., Saraf, M.N. (1999). Membrane stabilization activity—A possible mechanism of action for the anti-inflammatory activity of Cedrusdeodora wood oil. Fitoterapia, 251–257.
Sun, J., Zhang, G., Zhang, Y., Nan, N., Sun, X., Yu, M., Wang, H., Li, J., Wang, X. (2016). Spatholobus suberectus Column Extract Inhibits Estrogen Receptor Positive Breast Cancer via Suppressing ER MAPK PI3K/AKT Pathway. Evidenced Based Complementary and Alternative Medicine, 2016, 1-13.
Wang, F., Gomez-Sintes, R., Boya, P. (2018). Lysosomal Membrane Permeabilization and Cell Death. Traffic 19: 918-931. DOI: 10.1111/tra.12613
Wisastra, R, Dekker, F.J. (2014). Inflammation, cancer and oxidative lipoxygenase activity are intimately linked. Cancers (Basel), 6, 1500–1521. DOI: 10.3390/cancers6031500.
Wu, H. (1996). Affecting the activity of soybean lipoxygenase-1. J. Mol. Graph, 14, 331–337.
Yesmin, S., Paul, A., Nazl, T., Rahman, A., Akhter, S.F., Wahed, M.I.I., Emran, T. Siddiqui, S.A. (2020). Membrane stabilization as a mechanism of the anti-inflammatory activity of ethanolic root extract of Choi (Piper chaba). Clinical Phytoscience, 6, 59 https://doi.org/10.1186/s40816-020-00207-7
Refbacks
- There are currently no refbacks.
This work is licensed under a Creative Commons Attribution 4.0 International License.