The Effects of Dose Rhizoctonia Binucleat (BNR) and Phosphorus to Nitrate Reductase Activity (NRA) and Chlorophyll of Vanilla Seedling (Vanilla planifolia Andrews)

Haryuni Haryuni(1), Tyas Soemarah Kurnia Dewi(2),

DOI: https://doi.org/10.15294/biosaintifika.v8i2.6328


(1) Faculty of Agriculture, Tunas Pembangunan University at Surakarta Balekambang Lor street .1, Surakarta 57139. Center of Java, Indonesia
(2) Faculty of Agriculture, Tunas Pembangunan University at Surakarta Balekambang Lor street .1, Surakarta 57139. Center of Java, Indonesia

Abstract

Vanilla (Vanilla planifolia Andrews) is one of the important exported commodities in Indonesia. Indonesia is one of top five major vanilla exporters in the world, that produce the high quality of Indonesian vanilla with high vanillin content (2.75%). The aims of this research were to determine the effects of dose binukleat Rhizoctonia (BNR) and phosphorus as well as the interaction of the nitrate reductase activity (NRA) and chlorophyll of the vanilla seedling (Vanilla planifolia Andrew). Method in this research used completely randomized factorial design, by involving two factors (dose of BNR inoculation and Phosphor). The first factor is without inoculation and inoculation BNR (M0, M1, M2, M3) wich consists of (0,5, 10, 15) g/polybag, the second factor is the dose of phosphorus fertilizer (P0, P1, P2, P3) which consists of (0, 3, 6, 9) g/polibag. The results showed that the inoculation dose of BNR and doses of phosphorus not significant and lower levels of NRA and chlorophyll while the interaction dose of BNR and phosphorus significantly and increase levels of NRA and chlorophyll of vanilla seedling. Nitrate Reductase Activity and chlorophyll has important role in metabolism process as a plant growth indicator.

How to Cite

Haryuni, H., & Dewi, T. S. K. (2016). The Effects of Dose Rhizoctonia Binucleat (BNR) and Phosphorus to Nitrate Reductase Activity (NRA) and Chlorophyll of Vanilla Seedling (Vanilla planifolia Andrews). Biosaintifika: Journal of Biology & Biology Education, 8(2), 141-147.

Keywords

Binucleate Rhizoctonia; nitrat reductase activity; chlorophyll; phosphorus; vanilla

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References

Ai. N. S. & Banyo, Y. (2011) Leaf Chlorophyll Concentration as Indicators Water Shortage In scientific, Journal Crop Science. 11(2), 166-173.

Alnopri. (2004). Optimized of nitrate reductase activity assay procedures mangosteen leaves, Bengkulu. Jurnal Deed Agrosia, 7(2), 62-66.

Brundrett, M. (2004). Diversity and classification of mycorrhizal associations. Biological Reviews, 79(3), 473-495.

Dwidjoseputro, D. (1994) Pigments Chlorophyll. Jakarta: Erlangga.

Fitriana, J., Pukan, K. K., & Herlina, L. (2011). Aktivitas Enzim Nitrat Reduktase Kedelai Kultivar Burangrang akibat Variasi Kadar Air Tanah pada Awal Pengisian Polong. Biosaintifika: Journal of Biology & Biology Education, 1(1),1-8

Ginting, R. C. B., Saraswati, R., & Husen, E. (2006) Organic Fertilizer and Biofertilizer. Research and Development of Land Resources. Bogor.

Hanafi, A. S., Sabrina T., & Guchi, H. (2009) Soil Biology and Ecology. University of Northern Sumatra.

Hapiza, M. R., Sabrina, T., & Marbun, P. (2014). Pengaruh Pemberian Limbah Cair Industri Tempe dan Mikoriza Terhadap Ketersediaan Hara n dan p Serta Produksi Jagung (Zea Mays L.) Pada Tanah Inceptisol. Agroekoteknologi, 2(3), 1098-1106.

Haryuni. (2012). Studies On Binucleate Rhizoctonia As A Mycorrhiza And Its Role In Increasing Vanila Seedling Resistance Toward Drought Stress (Vanilla planifolia Andrews) against Drought Stress. Dissertation. Yogyakarta: Universitas Gadjah Mada.

Hendriyani, I. S., & Setiari, N. (2009). Kandungan klorofil dan pertumbuhan kacang panjang (Vigna sinensis) pada tingkat penyediaan air yang berbeda. Jurnal Sains & Matematika, 17(3), 145-150.

Indradewa, D., Sastrowinoto, S., Notohadisuwarno, S., & Prabowo, H. (2004). Metabolisme Nitrogen pada Tanaman Kedelai yang Mendapat Genangan dalam Parit Nitrogen Metabolism of Soybean Under Saturated Soil Culture. Ilmu Pertanian, 11(2), 68-75.

Krishna, K. R. (2005). Mycorrhizas: a molecular analysis. Michigan: Science Publishers.

Latifa, I. C., & Anggarwulan, E. (2009). Nitrogen content, nitrate reductase activity, and biomass of kimpul (Xanthosoma sagittifolium) on shade and nitrogen fertilizer variation. Nusantara Bioscience, 1(2), 65-71.

Lea, J. P. & Leegood, R. C. (1993). Nitrogen metabolism. In Lea, J.P. and R.C. Leegood (eds.) Plant Biochemistry and Molecular Biology. New York: John Wiley and Sons.

Li, R. H., Guo, P. G., Michael, B., Stefania, G., & Salvatore, C. (2006). Evaluation of chlorophyll content and fluorescence parameters as indicators of drought tolerance in barley. Agricultural Sciences in China, 5(10), 751-757.

McCashin, B. G. (2000). Induction of nitrate reductase in plant shoots. Tested studies for laboratory teaching. SJ Karcher ed, 21, 193-224.

Miswar, M. (2013, October). Respon Enzim Metabolisme Senyawa Nitrogen Pada Tanaman Tembakau Transgenik Yang Membawa Gen Sucrose Phosphate Synthase (Sps) Tebu (Saccharum officinarum L.). In Prosiding Seminar Biologi (Vol. 10, No. 2).

Nikoaloau, N. N., Karagiannidis, S., Koundouras, & Fysarakis, I. (2002). Effect of Different P Sources in Soil on Increasing Grows and Mineral Uptake of Mychorrizal finivera Vitis L. (cv Victoria) vines. Vigne Vin Sci Int, 36, 195-204.

Novi & Rizki (2014) The level of colonization Rooting Seed Banana Males inoculated with Multiple Dose inoculant Mycorrhizae Fungi Fungi And Giving Phosphate Lama. Journal Pelangi, 6(2), 99-108.

Osorio, A. I., Osorio Vega, N. W., Diez, M. C., & Moreno, F. H. (2014). Nutrient status and vegetative growth of Vanilla planifolia Jacks plants as affected by fertilization and organic substrate composition. Acta Agronómica, 63(4), 326-334.

Peni. D. K., Solichatun. I., & Anggarwulan, E. (2004) Growth, content of chrorophyll-carotenoids, saponins, and activity of nitrate reductase of Acalypha (Acalyphaindica L.) under different tea concentrations of gibberelic acid (GA3). Biopharmaceutical, 2(1), 1-8.

Purwandani, D. (2014). The Growth, Chlorophyll Content and Nitratre Reductase Activity of Sorghum bicolor (L). Moench Chromium Under Stress Condition. Essay. Salatiga: Universitas Kristen Satya Wacana

Rahardjo, M., Rosita, S. M. D., Sudiarto, & Hernani. (2000). Productivity and levels of flavonoids simpliciatempuyung (Sonchusarvensis L.) obtained under various conditions of water stress. News Industrial Crops Research and Development, 6(2), 1-3.

Rismunandar & Sukma, E. S. (2002) Planting Vanilla. Bogor: Governmental Spreader.

Sanjotha, P., Mahantesh, P., & Patil, C. S. (2011). Isolation and screening of efficiency of phosphate solubilizing microbes.International Journal of Microbiology Research, 3(1), 56.

Song, H. (2005). Effects of VAM on host plant in the condition of drought stress and its mechanisms. Electronic Journal of Biology, 1(3), 44-48.

Sufardi, S., Syakur, S., & Karnilawati, K. (2013). Amelioran Organik dan Mikoriza Meningkatkan Status Fosfat Tanah dan Hasil Jagung pada Tanah Andisol. Jurnal Agrista, 17(1), 1-11.

Vajpayee, P., Tripathi, R. D., Rai, U. N., Ali, M. B., & Singh, S. N. (2000). Chromium (VI) accumulation reduces chlorophyll biosynthesis, nitrate reductase activity and protein content in Nymphaea alba L. Chemosphere,41(7), 1075-1082.

Van der Mescht, A., & De Ronde, J. A. (1999). Chlorophyll fluorescence and chlorophyll content as a measure of drought tolerance in... South African journal of science, 95(9), 407-412.

Zhao, Q., Wang, H., Zhu, Z., Song, Y., & Yu, H. (2015). Effects of Bacillus cereus F-6 on Promoting Vanilla (Vanilla planifolia Andrews.) Plant Growth and Controlling Stem and Root Rot Disease. Agricultural Sciences, 6(9), 1068.

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