Microbial Succession and Chemical Characteristics in Fermentation of Ambonese Arrack (Sopi), Traditional Beverage from Maluku

Ferymon Mahulette, Dea Indriani Astuti


Ambonese arrack is one of the traditional fermented beverage product in Maluku, Indonesia. The microbiological research of this beverage that is processed using coconut sap as raw material has never been done before. The research aimed to analyze the microbial succession and chemical characteristics during fermentation of Ambonese arrack. The sample of coconut sap was taken from traditional producer in Mahia village, Ambon. The dominant microbes in the fermentation of Ambonese arrack were Pichia polymorpha and Kloeckera javanica. The highest numbers of these two microbes was obtained after 15 hours fermentation (9.6 log Cfu/mL and 9.9 Cfu/mL, respectively). The sugar content decreased from 593.3 mg/L to 474.3 mg/L, whereas ethanol content increased from 0.0018 g/L to 0.0100 g/L. The pH value decreased from 4.70 to 3.10. The research has isolated indigenous microbes in Ambonese arrack fermentation which was considered as novelty. The bacteria that play a role in fermentation can be used as a starter in the fermentation of various beverage products, especially Ambonese arrack. The results of this research can improve the quality of this fermentation product in the future.


Ambonese arrack; Coconut Sap; Ethanol Content; Microbial Succession

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Albertin, W., Setati, M.E., Miot-Sertier, C., Mostert, T., Colonna, B., Coulon, J., Girard, P., Moine, V., Pillet, M., Salin, F., Bely, M., Divol, B. & Masneuf-Pomarede, I. (2016). Hanseniaspora ovarum from winemaking environment show spatial and temporal genetic clustering. Front Microbiol, 6, 1-16

Albrecht, A.G., Peuckert, F., Landmann, H., Miethke, M., Seubert, A. & Marahiel, M.A. (2011). Mecha-nistic characterization of sulfur transfer from cysteine desulfurase SufS to the iron-sulfur scaffold SufU in Bacillus subtilis. FEBS Letters, 585, 465-470

Asakai, T. & Hioki, A. (2011). Investigation of iodine liberation process in redox titration of potassium iodate with sodium thiosulfate. Anal Chim Acta, 689, 34-38

Barlianti, V., Dahnum, D., Muryanto., Triwahyuni, E., Aristiawan, Y. & Sudiyani, Y. (2015). Enzymatic hydrolysis of oil palm empty fruit bunch to produce reducing sugar and its kinetic. Menara Perkebunan, 83(1), 37-43

Bekker, M.Z., Smith, M.E., Smith, P.A. & Wilkes, E.N. (2016) Formation hydrogen sulfide in wine: interactions between copper and sulfur dioxide. Molecules, 21, 1-17

Belda, I., Ruiz, J., Esteban-Fernandez, A., Navascues, E., Marquina, D., Santos, A. & Moreno-Arribas, V. (2017). Microbial contribution to wine aroma and its intended use for wine quality improvement. Molecules, 22, 1-29

Chandrasekhar, K., Sreevani, S., Seshapani, P. & Pramodhakumari, J (2012). A review on palm wine. Int J Res Biol Sci, 2(1), 33-38

Chandrasena, G., Keerthipala, A.P. & Walker, G.M. (2006). Isolation and characterization of Sri Lankan yeast germplasm and its evaluation for alcohol production. J Inst Brew, 112(4), 302-307

Chaves-Lopez, C., Serio, A., Grande-Tovar, C.D., Cuervo-Mulet, R., Delgado-Ospina, J. & Paparella, A. (2014). Tradition fermented foods and bev-erages from a microbiological and nutritional perspective: the Colombian heritage. Comp Rev Food Sci Food Safe, 13, 1031-1048

Danmadami, R.N., Yabaya, A., Yahaya, O., Abra-ham, O.J., Bobai, M. & Orukotan, A.A. (2017). The efficiency of Saccharomyces cerevisiae strain isolated from palm wine in the production of Burukutu. Int J Res Grant, 5(11), 70-85

Escalante, A., Gomez, M.G., Hernandez, G., Aguilar, M.S.C., Munguia, A.L., Gosset, G. & Bolivar F. (2008). Analysis of bacterial community during the fermentation of pulque, a traditional Mexican alcoholic beverage using a polyphasic approach. Int J Food Microbiol, 124, 126-134.

Gbrikon, G.M., Ichor, T. & Omeche, E.T. (2016). Effect of bitter leaf extract (Vernonia amygdalina) on culturable microorganisms isolated from palm wine in Makurdi metropolis. Res J Microbiol, 11(4-5), 112-118

Gunawan, L.S. (2019). Korelasi rasio aspartate aminotransferase - alanine aminotrasferase dengan profil hematologi pada peminum alcohol. J Biomedika, 12(1), 26-40

Kalaiyarasi, K., Sangeetha, K. & Rajarajab, S. (2013). A comparative study on the microbial flora of the fresh sap from cut inflorescence and fermented sap (toddy) of Borrassus flabellifer Linn (palmyrah tree) and of Cocos nucifera Linn (coconut tree) to identify the microbial fermenters. Int J Res Pure Appl Microbiol, 3(3), 43-47

Kim, S.A., Kim, N.H., Lee, S.H., Hwang, I.G. & Rhee, M.S. (2014). Survival of foodborne pathogenic bacteria (Bacillus cereus, Escherichia coli 0157:H7, Salmonella enterica Serovar typhimurium, Staphylococcus aureus, and Listeria monocytogenes) and Bacillus cereus spores in fermented alcoholic beverages (beer and refined rice wine). J Food Prot, 77(3), 419-426

Kleijn, R.J., Buescher, J.M., Le Chat, L., Jules, M., Aymerich, S. & Sauer, U. (2010). Metabolic fluxes during strong carbon catabolite repression by malate in Bacillus subtilis. J Biol Chem, 285(3), 1587-1596

Law, S.V., Abu Bakar, F., Hashim, M. & Abdul Ha-mid, A. (2011). Popular fermented food and beverages in Southeast Asia. Int Food Res J. 18, 475-484

Le, H, D,T. & Le, V.V.M (2014). Effect of initial pH value of medium on the alcoholic fermentation performance of Saccharomyces cerevisiae cells immobilized on nipa leaf sheath pieces. Songklanakarin J Sci Technol, 36(6), 663-667

Nair, G.R. & Raja, S.S.S. (2018). Climate and soil properties influence species diversity of soil Bacillus community in India. Microbiol Insights, 11, 1-7

Nwaiwu, O. & Itumoh, M. (2017). Chemical contam-inant associated with palm wine from Nigeria are potential food safety hazards. Beverages, 3(16), 1-12

Ohimain, E.I. (2016). Methanol contamination in traditionally fermented alcoholic beverages: the microbial dimension. Springers plus, 5, 1-10

Okanlawon, B.M., Ogunbanwo, S.T. & Okunlola, A.O. (2010). Growth of Bacillus cereus isolated from some traditional condiments under different regimens. Afr J Biotechnol, 8(14), 2129-2135

Olowonibi, O.O. (2017). Isolation and characteriza-tion of palm wine strain of Saccharomyces cerevisiae potentially useful as bakery yeast. Eur J Exp Biol, 7(2), 1-13

Roosdiana, A., Prasetyawan, S., Mahdi, C. & Su-trisno. (2013). Production and characterization of Bacillus firmus pectinase. J Pure App Chem Res, 2(1), 35-41

Sabri, A., Leroy, P., Haubruge, E., Hance, T., Frere, I., Destain, J. & Thonart, P. (2011). Isolation, pure culture and characterization of Serratia symbiotic asp. Nov., the R-type of secondary endosymbiont of black bean aphid Aphis fabae. Int J Syst Evol Microbiol, 61, 2081-2088

Sahusilawane, A.M., Uluputty, M.R., Kembauw, E. & Joko, S.W. (2015). Hapa, suatu kearifan local suku Meher di Pulau Kisar dalam menjaga ketahanan pangan. J Penel Kesejah Sos. 14(3), 305-316.

Sudha, R., Niral, V., Hebber, K.B. & Samsudeen, (2019). Coconut inflorescence sap. Curr Sci, 116(11), 1809-1817

Vengadaramana, A., Uthayasooriyan, M., Sittampa-lam, T., Razeek, N. & Kapilan R. (2016). The characterization of amylolytic enzyme present in fermented sweet sap of palmyrah. J App Biol Biotechnol, 4(3), 20-23

Zhu, Y., Zhang, F., Zhang, C., Yang, L., Fan, G., Xu, Y., Sun, B. & Li, X. (2018). Dynamic microbial succession of Shanxi aged vinegar and its correlation with flavor metabolites during different stages of acetic acid fermentation. Sci Rep, 8, 1-10.

DOI: https://doi.org/10.15294/biosaintifika.v12i2.23791


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