Ecological Studies of Oryctes rhinoceros Larvae Controlled by Metarhizium anisopliae and Enthomopatogenic Nematodes

D. R. Indriyanti, R. Rahmawati, B. Priyono, M. Slamet, F. Z. Huyop

Abstract

Oryctes rhinoceros L. (Coleoptera: Scarabaeidae) is a pest of coconut plant. Metarhizium anisopliae and Enthomopatogenic Nematodes are biological control agents. The purpose of this study was to determine the ecology of insect pests controlled by fungi Metarhizium anisopliae (MET), Enthomopatogenic Nematodes (NEP) and and the mixture of MET and NEP for controlling O. rhinoceros larvae on the field. M. anisopliae used was in the form of kaolin powder formulation (WP). The nematodes used were Heterorhabditios sp. Nematodes formulation was in the form of liquid with sponge medium contained 10 x 106 NEP in every pack. The study used Randomized Block Design with 3 types of treatment and 10 times repetition on 10 larvae. The result of six weeks treatment showed that the highest larval mortality was obtained from the treatment with the mixture of MET and NEP. Biological control using two biological agents (MET and NEP) is better than only MET or NEP. The benefit of research is for the recommendation of O. rhinoceros larvae control.

Keywords

application of Metarhizium anisopliae, Enthomopatogenic Nematodes, Oryctes rhinoceros, biological control.

Full Text:

PDF

References

Allou, K., Morin, J. P., Kouassi, P., & N, F. H. (2006). Oryctes monoceros Trapping with Synthetic Pheromone and Palm Material in Ivory Coast. Journal of Chemical Ecology, 32(8), 1743–1754.

Agali, A.E.A., Mohammed, E.A.E.R., Hassan, A.E.W. (2017). Effect of entomopathogenic fungi Metarhizium anisopliae var acridum and Beauveria bassiana on survival of Anopheles arabiensis Patton and Culex quinquefasciatus Say mosquito larvae. Sudan Journal of Science (SJS) 9(2), 26- 41.

Adams. B.J & Nguyen, K.B. (2002). Taxonomy and Systematics. Pp 1-28 in: R. Gaugler (Ed). Entomopathogenic Nematology. CAB International, Wallingford, Oxford.

Amalia, A.V & Yusa, M.H. (2018). Control pest of leaf caterpillars (Plutella xylostella) in delima rose apples using soursop leaf extract (Annona muricata). Jurnal Pendidikan IPA Indonesia. 7(1), 1-8.

Ansari, M.A,. Shah, F.A. & Butt T.M. (2008). Combined use of entomopathogenic nematodes and Metarhizium anisopliae as a new approach for black vine weevil, Otiorhynchus sulcatus, control. Journal compilatiom The Netherlands Entomological Society. 129: 340- 347.

Bedford, G. O. (2013). Long-term Reduction in Damage by Rhinoceros Beetle Oryctes rhinoceros (L.) (Coleoptera: Scarabaeidae: Dysnastinae) to Coconut palm at Oryctes Nudivirus release Site on Viti Levu, Fiji. 8 (49): 6422-6425.

Boemare, N.E., Lanmond & Mauleon, H. (1996). The entomopathogenic nematodes Bacterium complex, biology, life cycle and vertebrate safety. Journal of Biocontrol Science and Technology 6 (1): 333-346.

Gusmara, B.H. (2011). Pembuatan dan pengujian formula Metahizium majus UICC 295 dengan media pembawa substrat beras (Oryza sativa) terhadap larva Oryctes rhinoceros. (Skripsi). Depok: Universitas Indonesia.

Indriyanti, D.R., Damayanti, I.B., Setiati, N., Maretta, Y.A. (2018a). Mortality and tissue damage of Oryctes rhinoceros larvae infected by Metarhizium anisopliae. ARPN, 13(6), 2279- 2286.

Indriyanti, D.R., Lutfiana, J.E., Widiyaningrum, P., Susilowati, E and Slamet, M. (2018b). Aggregation pheromones for monitoring the coconut rhinoceros beetle (Oryctes rhinoceros) in Jerukwangi Village, Jepara, Indonesia. J. Phys.: Conf. Ser. 983 012177.

Indriyanti, D.R., Putri,R.I.P., Widiyaningrum, P & Herlina, L. (2017a). Density, viability conidia and symptoms of Metarhizium anisopliae infection on Oryctes rhinoceros larvae. Journal of Physics: Conf. Series 824 (2017) 012058 doi:10.1088/1742-6596/824/1/012058.

Indriyanti, D.R., Nuraini, I., Slamet, M. (2017b). The Effect of Water Content of Medium Containing Oryctes rhinoceros Larvae on Metarhizium anisopliae Pathogenicity. Biosaintifika 9 (2): 363-369.

Indriyanti, D.R., Widiyaningrum, P., Haryuni, Slamet, M. & Maretta,Y.A. (2017c). Effectiveness of Metarhizium anisopliae and Entomopathogenic Nematodes to Control Oryctes rhinoceros Larvae in the Rainy Season. Pakistan Journal of Biological Sciences, 20(7): 320-327.

Indriyanti, D.R & Widiyaningrum, P. (2014). Exploration of Entomopatogenic nematodes from soils as Biological Agents of Insect Pests. Makalah diseminarkan di Pertemuan Seminar Nasional Himpunan Profesi HPTI, PEI, dan PFI Jawa Timur. Di UPN Veteran Jawa Timur 19 Maret 2014.

Kaya, M.G. (1993). EfficiacyAgainst Soil-Inhibitting Insect Pests. In: Gauler Kaya H K. (Ed) Entomophatogenic Nematodes in Biological Control. Florida: CRC Press.

Manan, A & Suyanto, A. (2009). Kemempanan Isolat Lokal Nematoda Entomopatogen Steinernema carpocapsae Poinar Untuk Pengendalian Hama Ulat Grayak (Spodoptera litura F.). Jurnal Pembangunan Pedesaan 9(1): 35-42.

Manjeri G, Muhamad R & Tan SG. (2014). Oryctyes rhinoceros Beetles, An Oil Palm Pest In Malaysia. Annual Research & Review in Biology, 4(22): 3429-3439.

Pracaya. 2004. Hama Penyakit Tanaman. Depok: Penebar Swadaya.

Prayogo, Y., Wedanimbi, T & Marwoto. (2005). Pemanfaatan cendawan entomopatogen Metarhizium anisopliae untuk mengendalikan ulat grayak Spodoptera litura pada kedelai. Jurnal Penelitian dan Pengembangan Pertanian 94 (1): 19-26.

Pujiastuti, Y. (2010). Tingkat Populasi dan Kebugaran Oryctes rhinoceros L Coleoptera: Scarabaeidae di Perkebunan Kelapa Sawit (Elaeis Guineensis Jacq). Prosiding Seminar Nasional Penelitian Bidang Pertania. Vol 2.

Sucipto. (2008.) Persistensi Nematoda entomopatogen Heterorhabditis (All Strain) isolate local Madura terhadap pengendalian rayap tanah Macrotermes sp. (Isoptera: Termitidae) di lapang. Jurnal Embryo. 5 (2): 193-208.

Sucipto. (2009). Nematoda Entomopatogen Heterorhabditis Isolat Lokal Madura sebagai Pengendalian Hayati Penting Tanaman Hortikultura yang Ramah Lingkungan. Agrovigor 2 (1): 47-53.

Suyanto, A., Srimurni, E & Djuharyanto, T. (2012). Perkembangan Larva Serangga Hama Kumbang Badak (Oryctes rhinoceros L.) pada Berbagai Konsentrasi Isolat Nematoda Entomopatogen Heterorhabditis sp. Prosiding Seminar Nasional ISBN: 978-979-9204-79-0; Purwokerto.

Weather and climate. (2016). Average, Monthly, Precipitation, Rainfall Jepara Central Java. https://weather-and-climate.com/average-monthly-precipitation-Rainfall,jepara-central-java-id,Indonesia.

Windarti, P.W. (2010). The effect of Metarhizium anisopliae fungi on mortality of Aedes aegypti larvae. Jurnal Natural 10(1):31-35.

Refbacks

  • There are currently no refbacks.