Canavalia ensiformis Protein Extract Effect Toward Serum Lipid Profile of Hypercholesterolemic Sprague Dawley Rat

Mira Dian Naufalina, Muchlis AU Sofro, Gemala Anjani

Abstract

Riskesdas 2013 showed the prevalence of abnormal total cholesterol levels in Indonesia is 35.9%, low HDL cholesterol is 22.9%, high LDL cholesterol is 15.9%, and high triglyceride is 11.9%. This study aimed to prove the effect of jack bean protein extract toward serum lipid profile of a hypercholesterolemic rat. This was a true experimental study conducted in September 2016 with post-only control group design. Twenty four male Sprague Dawley rats were randomly classified into 4 different groups; control negative group (C-), control positive group (C+), first experimental group (X1) which was given jack bean extract 4g/200g of body weight of the rat per day, and second experimental group (X2) which was given jack bean extracts 6g/200g of body weight of the rat per day. Treatment for 2 weeks showed a significant effect. Total cholesterol, LDL cholesterol, and triglycerides levels of groups with protein jack bean extract administration were lower than the positive control group (p <0.05). Increasing doses of jack bean extracts was linear with serum lipid profile improvement of Sprague Dawley Rat. These result indicated the positive effect of jack bean protein extract toward serum lipid profile of hypercholesterolemic Sprague Dawley Rat.

Keywords

Jack bean protein extract, Hypercholesterolaemia, Serum lipid profile, Sprague Dawley Rat

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References

Ajeigbe SO, Mohammed AK, Yahaya IA dan Oyelowo AO. 2012. Effect of Processing Techniques on Levels of Minerals and Antinutritional Factors of Canavalia ensiformis. Pak J Nutr 11: 1121-1124).

Ananta F. 2016. Pengaruh Pemberian Kecambah Kacang kedelai terhadap Kadar Profil Lipid dan Perlemakan Hati Studi Eksperimental pada Tikus Sprague Dawley Hiperkolesterolemik. Tesis. Universitas Diponegoro.

Badan Standardisasi Nasional. 1992. Cara Uji Makanan dan Minuman (SNI 01-. 2891-1992). BSN. Jakarta.

Bazzano LA, Thompson AM, Tees MT, Nguyen, Winham DM. 2011. Non-Soy Legume Consumption Lowers Cholesterol Levels: A Meta-Analysis of Randomized Controlled Trials. Nutr Metab Cardiovasc Dis.;21(2): 94103.

Bloedon LT, Philippe O, Szapary PO: Flaxseed and cardiovascular risk. Nutr Rev 2004, 62:1827.

Cho SJ, Juillerat MA, Lee CH. 2007. Cholesterol lowering mechanism of soybean protein hydrolysate. J Agric Food Chem 55:10599-10604.

Fakhrzadeh H, Tabatabaei Malazy O. 2012. Dyslipidemia and cardiovascular disease. In: Kelishadi R, editor. Dyslipidemia: From Prevention to Treatment. 1st ed. Croatia: InTech; p. 303 20.

Garba U, Kaur S. 2014. Review-Protein Isolate: Production, Functional Properties and Application. International Research Journal and Chemistry.;4; 22-36.

Gropper SS, Smith JL, Groff JL. 2009. Advanced Nutrition and Human Metabolism.5th ed. Belmont: Wadsworth;.p 115.

Kayashima T, Okazaki Y, Katayama T, Hori K. 2005. Dietery Lectin Lowers Serum Cholesterol and Raises Fecal Neutral Sterols in Cholesterol-Fed Rats. J Nutr Sci Vitaminol.;51(5):343-8.

Legowo AM. Nuewanto, Sutantyo. 2005. Analisis Pangan. Badan penerbit Universitas Diponegoro Semarang.

Lenkala, p. 2014. Morphological characterization and evaluation of jack bean (Canavalia ensiformis (l.) Dc.) Genotypes for yield and quality characters. Doctoral dissertation, dr. YSR Horticultural University, India.

Maharana L. 2013. Experimantal. ietd.iflibnet.ac.id. (Accessed Feb 2016).

Marfo EK, Wallace P, Timpo G, Simpson BK. 1990. Cholesterol lowering effect of jackbean (Canavalia ensiformis) seed protein. Gen Pharmac.;21(5): 753-57.

Medichem Middle East. 2010a. Manual Procedure Cholesterol CHOD-PAP Enzymatic Colorimetric Methode. Medichem Middle East Syiria.

Medichem Middle East. 2010b. Manual Procedure LDL-Cholesterol Precipitation Method. Medichem Middle East Syiria.

Medichem Middle East. 2010c. Manual Procedure Triglycerides GPO/PAP Enzymatic Colorimetric Method. 1st Edition. http://www.medichem-me.com/Method/english/Triglycerides mono .pdf. (accessed 21 Mar 2016)

National Institutes of Health. 2002. Third Report of the National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel III) final report. Circulation. ;106:3143421.

Nelson RH. 2013. Hyperlipidemia as a Risk Factor for Cardiovascular Disease. Prim Care.;40(1): 195211.

Nimenibo-Uadia R. 2003. Effect of Aqueous Extract of Canavalia ensiformis Seeds On Hyperlipidaemia And Hyperketonaemia in Alloxan-Induced Diabetic Rats. Nigerian Society for Experimental Biology. Jun; 15(1): 7-15.

Nurfitriani A. 2014. Isolasi dan Karakterisasi sifat Fungsional Protein Serta Pemanfaatan Kacang Koro Pedang (Canavalia ensiformis) sebagai Bahan Dasar dalam Pembuatan Tofu. Tesis. Universitas Hassanuddin.

Pallav Segupta. 2013. The Laboratory Rat: Relating Its Age with Humans. Int.J.Prev.Med. June;4(6):624-630.

Rigamonti E, Parolini C, Marchesi M, Diani E, Brambilla S, Sirtori CR, Chiesa G. 2010. Hypolipidemic Effect of Dietary Pea Proteins: Impact on Gene Regulating Hepatic Lipid Metabolism. Mol Nutr Food Res 54: S24-S30.

Sastroasmoro S. dan Ismael S. 2008. Dasar-dasar Metodologi Penelitian Klinis. Bagian Ilmu Kesehatan Anak Fakultas Kedokteran Universitas Indonesia, CV Sagung seto: Jakarta.: 109-25.

Shrestha S, Freake HC, McGrane MM, et al. 2007. A combination of psyllium and plant sterols alters lipoprotein metabolism in hypercholesterolemic subjects by modifying the intravascular processing of lipoproteins and increasing LDL uptake. J Nutr; 137:116570.

Spielmann J. Stangl G, Eder K. 2008. Dietary pea protein stimulates bile acid excretion and lowers hepatic cholesterol concentration in rats. J Anim Physiol Anim Nutr Berl 92:683-693.

Sridhar KR, Seena S. 2006. Nutritional and Antinutritional Significance of Four Unconventional Legumes of The Genus Canavalia A comparative study. Food Chem 99: 26788.

Subagio A. 2016. Strategi Pencapaian Swasembada Kedelai dengan Pengembangan Sumber Protein Nabati Alternatif. JURNAL PANGAN. Jun 15;19(2):127-34.

Tall AR. 2010. Functions of cholesterol ester transfer protein and relationship to coronary artery disease risk. J Clin Lipidol. 4(5): 38993.

Wahyuningsih SB, Saddewisasi W. 2013. Pemanfaatan Koro Pedang pada Aplikasi Produk Pangan dan Analisis Ekonominya. Riptek;7(2): 1-10.

Wiklund O, Pirazzi C, Romeo S. 2013. Monitoring of Lipids, Enzymes, and Creatine Kinase in Patients on Lipid-Lowering Drug Therapy. Curr Cardiol Rep.;15:397.

World Health Organization. 2015. Cardiovascular Disease (CVDs). Available at http://www.who.int/mediacentre/factsheets/fs317/en/

World Heart Federation. 2008. Death Due to Cardiovascular Disease. Available at http://www.world-heart-federation.org/cardiovascular-health/global-facts-map/

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