Detection of Dengue Virus Transovarial Transmission in Dengue Hemorrhagic Fever Endemic Areas

Malik Saepudin(1), Heru Subaris Kasjono(2), Martini Martini(3),


(1) 
(2) Poltekkes Kemenkes Yogyakarta
(3) 

Abstract

Dengue virus is a group of RNA viruses that are highly pathogenic in humans and spread quickly through the bites of Aedes aegypti and Aedes albopictus mosquitoes, especially in tropical countries. More than half a billion out of 100 countries worldwide are at serious risk of dengue virus infection. Vector surveillance activities with Ovitrap and detection of dengue virus types in Aedes aegypti and Aedes albopictus have never been carried out in Pontianak City. It is important in early alert systems at transmission foci. The purpose of this study was to prove the transovarial transmission of dengue virus in Aedes aegypti and Aedes albopictus mosquitoes with a transovarial transmission index (TTI) in endemic areas in Pontianak City, West Kalimantan. The method used in this research is descriptive observational, viral examination method with immunocytochemistry streptavidin-biotin peroxidase complex (ISBPC) and Polymerase Chain Reaction Transcription Reaction (PCR) aimed at proving the presence of transovarial transmission of dengue virus in the same period. The conclusion in this study is that there is evidence of transovarial transmission of dengue virus in Aedes mosquitoes in endemic areas by 29.3% in Sungai Jawi Dalam sub-district, West Pontianak sub-district, and 39.6% in Batu Layang sub-district, North Pontianak sub-district, mosquito density from the results of the Ovitrap Index measurement (OI) in Batu Layang Village is denser, namely 41.3%, compared to Sungai Jawi Village, which is 38.22% and has succeeded in identifying the type of dengue virus, namely the Dengue virus strain, in the two research locations.

Keywords

transmisi transovarial,virus dengue, Aedes aegypti

Full Text:

PDF

References

Achmadi, U.F., 2012. Manajemen Demam Berdarah Berbasis Wilayah, Buletin Jendela Epidemiologi Puslitbang Ekologi Dan Statistik Kesehatan. Jakarta: Kementerian Kesehatan RI.

Da-Costa, C.F., dos-Passos, R.A., Lima, J.B.P., Roque, R.A., Sampaio, V.d-S., Campolina, T.B., Secundino, N.F.C., & Pimenta, P.F.P., 2017. Transovarial Transmission of DENV in Aedes Aegypti in the Amazon Basin: A Local Model of Xenomonitoring. Parasites and Vectors 10(1), pp.1–9.

Da-Cruz, L.C.d-T.A., Serra, O.P., Leal-Santos, F.A., Ribeiro, A.L.M., Slhessarenko, R.D., dos-Santos, M.A., 2015. Natural Transovarial Transmission of Dengue Virus 4 in Aedes Aegypti from Cuiabá, State of Mato Grosso, Brazil. Revista da Sociedade Brasileira de Medicina Tropical, 48(1), pp.18–25.

Dinas Kesehatan Kota Pontianak., 2017. Profil Kesehatan Kota Pontianak Tahun 2017.

Espinosa, M., Giamperetti, S., Abril, M., & Seijo, A., 2014. Transmisión Vertical de Virus Dengue En Aedes Aegypti, Capturados En Puerto Iguazú, Misiones, Argentina. Revista do Instituto de Medicina Tropical de Sao Paulo, 56(2), pp.165–67.

Ferreira-De-Lima., Henrique, V., & Lima-Camara, T.N., 2018. Natural Vertical Transmission of Dengue Virus in Aedes Aegypti and Aedes Albopictus: A Systematic Review. Parasites and Vectors, 11(1), pp.1–8.

Fuadzy, H., Widawati, M., Astuti, E.P., Prasetyowati, H., Hendri, J., Nurindra, R.W., & Hodijah, D.N., 2020. Risk Factors Associated with Dengue Incidence in Bandung, Indonesia: A Household Based Case-Control Study. Health Science Journal of Indonesia, 11(1), pp.45–51.

George, L., Lenhart, A., Toledo, J., Lazaro, A., Han, W.W., Velayudhan, R., Ranzinger, S.R., & Horstick, O., 2015. Community-Effectiveness of Temephos for Dengue Vector Control: A Systematic Literature Review. PLoS Neglected Tropical Diseases, 9(9).

Halsey, E.S., Marks, M.A., Gotuzzo, E., Fiestas, V., Suarez, L., Vargas, J., Aguayo, N., Madrid, C., Vimos, C., Kochel, T.J., & Laguna-Torres, V.A., 2012. Correlation of Serotype-Specific Dengue Virus Infection with Clinical Manifestations. PLoS Neglected Tropical Diseases, 6(5).

Heath, C.J., Grossi-Soyster, E.N., Ndenga, B.A., Mutuku, F.M., Sahoo, M.K., Ngugi, H.N., Mbakaya, J.O., Siema, P., Kitron, U., Zahiri, N., Hortion, J., Waggoner, J.J., King, C.H., Pinsky, B.A., & LaBeaud, A.D., 2020. Evidence of Transovarial Transmission of Chikungunya and Dengue Viruses in Fieldcaught Mosquitoes in Kenya. PLoS Neglected Tropical Diseases, 14(6), pp.1–17.

Hikmawati, I., Wahjono, H., Martini., Hadisaputro, S., Darmana, E., Djati, K.R.M., Mardihusodo, S.J., & Umniyati, S.R., 2020. Rapid Detection of Dengue Virus Transovarial Transmition from Nature and Artificial Inoculation. Journal of Critical Reviews, 7(1), pp.41–47.

Matangkasombut, P., Manopwisedjaroen, K., Pitabut, N., Thaloengsok, S., Suraamornkul, S., Yingtaweesak, T., Duong, V., Sakuntabhai, A., Paul, R., & Singhasivanon, P., 2020. Dengue Viremia Kinetics in Asymptomatic and Symptomatic Infection. International Journal of Infectious Diseases, 101, pp.90–97.

Rahayu, A., Saraswati, U., Supriyati, E., Kumalawati, D.A., Hermantara, R., Rovik, A., Daniwijaya, E.W., Fitriana, I., Setyawan, S., Ahmad, R.A., Wardana, D.S., Indriani, C., Utarini, A., Tantowijoyo, W., & Arguni, E., 2019. Prevalence and Distribution of Dengue Virus in Aedes Aegypti in Yogyakarta City before Deployment of Wolbachia Infected Aedes Aegypti. International Journal of Environmental Research and Public Health, 16(10), pp.1–12.

Rosa, E., & Salmah, S., 2015. Detection of Transovarial Dengue Virus with RT-PCR in Aedes Albopictus ( Skuse ) Larvae Inhabiting Phytotelmata in Endemic DHF Areas in West Sumatra , Indonesia. American Journal of Infectious Diseases and Mircobiology, 3(1), pp.14–17.

Saepudin, M., 2011. Metodologi Kesehatan Masyarakat. Jakarta: Trans Info Media (TIM).

Saepudin, M., Hadisaputro, S., Suwondo, A., & Suhartono., 2017. The Effect of Rekattidiri Ovitrap towards Aedes Aegypti Larval Density. International Journal of Public Health Science (IJPHS), 6(1), pp.80–86.

Saepudin, M., Hadisaputro, S., & Umniyati, S.R., 2019. The Effect of Sticky Autocidal Mosquito Trap (SAMT) on the Index of Transovarial Transmission of Dengue Viruses. Indian Journal of Public Health Research & Development, 10(12), pp.1812–17.

Soo, K.M., Khalid, B., Ching, S.M., & Chee, H.Y., 2016. Meta-Analysis of Dengue Severity during Infection by Different Dengue Virus Serotypes in Primary and Secondary Infections. PLoS ONE, 11(5), pp.4–14.

Sorisi, A.M.H., Umniyati, S.R., Satoto, T.B.T., 2014. Transovarial Transmission Index of Dengue Virus on Aedes Aegypti and Aedes Albopictus Mosquitoes in Malalayang District in Manado, North Sulawesi, Indonesia. Tropical Medicine Journal, 1(2), pp.87–95.

Sucipto, C.D., 2012. Dengue Transovaria Virus Transmission Detection in the Aedes Aegypti Mosquito. Journal Of Health Science, XII, pp.121.

Sunardi, P., Kusnanto, H., Satoto, T.B.T., & Lazuardi, L., 2018. Prevalence of Dengue Virus Transovarial Transmission and DHF Incidence Rate in Grogol Sub-District of Sukoharjo District. Jurnal Medicoeticolegal dan Manajemen Rumah Sakit, 7(2), pp.102–7.

Utama, I.M.S., Lukman, N., Sukmawati, D.D., Alisjahbana, B., Alam, A., Murniati, D., Utama, I.M.G.D.L., Puspitasari, D., Kosasih, H., Laksono, I., Karyana, M., Karyanti, M.R.K., Hapsari, M.M.D.E.A.H., Meutia, N., Liang, C.J., Wulan, W.N., Lau, C.Y., & Parwati, K.T.M., 2019. Dengue Viral Infection in Indonesia: Epidemiology, Diagnostic Challenges, and Mutations from an Observational Cohort Study. Plos Neglected Tropical Diseases, 2019, pp.1–19.

Refbacks





Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.