Transovarial Transmission and Dengue Virus Serotypes in Aedes Aegypti In Kupang

Wanti Wanti, Oktovianus Sila, Irfan Irfan, Enni Sinaga


Dengue is an endemic disease in almost all provinces in Indonesia, including NTT pro-vince and Kupang City. DHF prevention and control efforts need to be accompanied by understanding of the epidemiological and entomological aspects. This study proves the presence of transovarial transmission and serotypes of Dengue virus in Ae. aegypti in areas with different endemics in Kupang City. The study was conducted in endemic, sporadic and Dengue-free villages. Samples from each location were 30 head squash Ae. aegypti females for examination of Dengue virus by IHC methods and 10 Ae. aegypti females for serotypes check of Dengue virus with RTPCR. Data presented in tables and images then analyzed descriptively. This study finds that transovarial infection rates 9.2% with serotype Dengue virus-1. As many 97.5% of respondents have heard about Dengue by most cadres resources (24.5%) and health workers (24%). Transovarial infection is found in endemic, sporadic and free areas so that the Dengue vector control needs to be done in the three regions with different endemicity.


Virus; Transovarial; Ae. aegypti; Dengu

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Achmadi, U.F. 2010. Manajemen Demam Berdarah Berbasis Wilayah. Buletin Jendela Epidemiologi, 2 : 15-20.

Angel, B., Joshi, V. 2008. Distribution and Seasonality of Vertical Transmitted Dengue Virus in Aedes mosquitoes in Arid and Semi-Arid Areas of Rajasthan, India. J Vector Borne Disease. 45 (3): 56-59.

Aryati, Wardhani P, Yohan B, Aksono EB, Sasmono T. 2012. Dengue Serotype Distribution in Surabaya in the Year 2012. Indonesian Journal of Clinical Pathology and Medical Laboratory. 19 (1) : 41-44.

Calderon-Arguedas, O., Avendano, A., Lopez-Sanchez, W., Troyo, A. 2010. Expansion of Aedes albopictus skull in Costa Rica. Rev. Ibero-Latinoam Parasitol. 69 (2): 220-222.

Cecilio, A.B., Campanelli, E.S., Souza, K.P.R., Figueiredo, L.B., Resende, M.C. 2009. Natural Vertical Transmission by Stegomyia albopicta as Dengue Vector in Brazil. Braz J Biol, 69 (1): 123-127.

Da Cruz, L.C.T.A., Serra, O.P., Leal-Santos, F.A., Ribeiro, A.L.M., Slhessarenko, R.D., dos Santos, M.A. 2014. Natural Tranovarial Transmission of Dengue Virus 4 in Aedes aegypti from Cuiaba, State of Mato Grooso, Brazil. Revista da Sociedade Brasileira de Medicina Tropical, 48 (1): 18-25.

Dinkes Kota Kupang. 2013. Profil Kesehatan Kota Kupang 2012. Kupang:Dinkes Kota Kupang.

Ditjen PP & PL. 2013. Pedoman Pengendalian Demam Berdarah Dengue di Indonesia. Kemenkes RI, Jakarta.

Espinosa M., Giamperetti, S., Abril, Marcelo, Seijo, A. 2014. Vertical Transmission of Dengue Virus in Aedes aegypti Collected. Rev Inst Med Trop SaoPaulo. 56(2): 165-167.

Guerdan, B.R. 2010. Dengue Fever/Dengue Hemorrhagic Fever. American Journal of Clinical Medicine. 7 (2): 51-53

Heyman, D.L. 2008. Control of Communicable Diseases Manual. Edisi ke 19. APHA.

Kemenkes RI. 2013. Profil Kesehatan Indonesia 2012. Jakarta: Kemenkes RI.

Luplertlop, N. 2014. Aedes Mosquito Salivary Immune peptides: Boost or Block Dengue Viral Infection. J of Coastal Life Medicine, 2 (2): 163-168.

Mardihusodo, S.J., Satoto, T.B.T., Mulyaningsih, B., Umniyati, S.R., Ernaningsih. 2007. Bukti Adanya Penularan Virus Dengue Secara Transovarial pada Nyamuk Aedes aegypti di Kota Yogyakarta. Simposium Nasional Aspek Biologi Molekuler, Patogenesis, Manajemen dan Pencegahan KLB. 16 Mei 2007. Yogyakarta.

Pusat Data dan Surveilans Epidemiologi. 2010. Demam Berdarah Dengue di Indonesia Tahun 1968 2009. Buletin Jendela Epidemiologi, 2 : 1-14.

Rohani, A., Asmalia, I., Zainal, S., Lee, H.L. 1997. Detection of Dengue Virus from Field Aedes aegypti and Aedes albopictus Adults and Larvae. Southeast Asian J Trop Med and Public Health, 28 (1): 138-141.

Rohani, A., Zamree, I., Joseph, R.T., Lee, H.L. 2008. Persistency of Transovarial Dengue Virus in Aedes aegypti (Linn.). Southeast Asian J Trop Med Publich Health, 39 (5): 813-816.

Satoto, T.T.T., Umniyati, S.R., Astuti, F.D., Wijayanti, N., Gavotte, L., Devaux, C., Frutor, R. 2014. Assesment of Vertical Dengue Virus Transmission in Aedes aegypti and Serotype Prevalence in Bantul, Indonesia. Asian Pacific J Trop Dis, 4(Suppl 2): S563-S568. Doi: 10.1016/S2222-1808(14)60677-0.

Satoto, T.B.T., Umniyati, S.R., Suardipa, A., Sintorini, M.M. 2013. Effects of Temperature, Relative Humidity, and Den-2 Virus Transovarial Infection on Viability of Aedes aegypti. Jurnal Kesmas, 7 (7): 331-336.

Sukowinarsih, T.E., Cahyati, W.H. 2011. Hubungan Sanitasi Rumah dengan Angka Bebas Jentik Aedes aegypti. Jurnal Kemas. 6 (1): 30-35.

Thongrungkiat, S., Maneekan, P., Wasinpiyamonkol, L., Prummongkol, S. 2011. Prospective Field Study of TRansovarial Dengue-Virus Transmission by Two Different Forms of Aedes aegypti in an Urban Area of Bangkok, Thailand. Journal of Vector Ecology, 36 (1): 147-152.

Umniyati, S.R. 2004. Preliminary Investigation on The Transovarial Transmission of Dengue Virus in The Population of Aedes aegypti in The Well. Seminar Peringatan Hari Nyamuk IV. 21 Agustus 2004. Surabaya.


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