Email this article The Expression Change of Mmp-8 and Collagen Type-2 Intracell in Lung Tissue Due to Electronic Smoke Exposure
(1) Universitas Surabaya
(2) Universitas Surabaya
(3) Universitas Surabaya
(4) Universitas Surabaya
Abstract
The number of electronic smokers has increased annually. Exposure to an electronic cigarette will increase free radicals in the body and result in oxidative stress causing lung tissue damage. The severity degree of lung tissue damage caused by electronic smoke exposure depends on the duration of electronic cigarette smoke exposure, and will affect Matrix Metalloproteinase-8 and collagen type-2 in the cells. The study aims to understand the change degree of Matrix Metalloproteinase-8 and collagen type-2in lung tissue due to electronic cigarette smoke exposure. This study applied the experimental method with a post control group design. The male Wistar rats were used as the animal models in this research to assess cell damage through the Matrix Metalloproteinase-8 expression and collagen type-2 in the lung tissue using immunohistochemical staining. Exposure to electronic smoke cigarettes was given to each group of animal models with the difference in amount and time duration. The expression of Matrix Metalloproteinase-8 indicated a significant increase due to electronic smoke exposure (ANOVA, p=0.000). Meanwhile the expression of collagen type-2 showed a significant decrease because of electronic smoke exposure (ANOVA, p=0.000). Besides, MMP-8 and collagen type-2 manifested relationship existence and strong impact (r=0.948, p=0.000). The negative impact of exposure to electric cigarette smoke causes increased expression of Matrix Metalloproteinase-8 and decreased expression of type-2 collagen in lung tissue.
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Amrock, S. M., Lee, L., & Weitzman, M., 2016. Perceptions of e-Cigarettes and Noncigarette Tobacco Products Among US Youth. Pediatrics, 138(5), e20154306.
Arulselvan, P., Fard, M.T., Tan, W.S., Gothai, S., Fakurazi, S., Norhaizan, M. E., & Kumar, S.S., 2016. Role of Antioxidants and Natural Products in Inflammation. Oxidative Medicine and Cellular Longevity, 2016, pp.1–15.
Asano, K., Shikama, Y., Shoji, N., Hirano, K., Suzaki, H., & Nakajima, H., 2010. Tiotropium bromide inhibits TGF-β-induced MMP production from lung fibroblasts by interfering with Smad and MAPK pathways in vitro. International Journal of Chronic Obstructive Pulmonary Disease, 5, pp.277–286.
Awadalla, N.J., Hegazy, A., Elmetwally, R.A., & Wahby, I., 2012. Occupational and Environmental Risk Factors for Idiopathic Pulmonary Fibrosis in Egypt: A Multicenter Case-control Study. The International Journal of Occupational and Environmental Medicine, 3(3), pp.107–116.
Basu, R.K., Donaworth, E., Siroky, B., Devarajan, P., & Wong, H.R., 2015. Loss of Matrix Metalloproteinase-8 is Associated with Worsened Recovery After Ischemic Kidney Injury. Renal Failure, 37(3), pp.469–475.
Canistro, D., Vivarelli, F., Cirillo, S., Marquillas, C.B., Lazzaretti, M., Laura, M., Paolini, M., 2017. E-cigarettes Induce Toxicological Effects that Can Raise the Cancer Risk, National Center for Biotechnology Information, 17(1), pp.1–9.
Chen, L., Deng, H., Cui, H., Fang, J., Zuo, Z., Deng, J., & Zhao, L., 2018. Inflammatory Responses and Inflammation-Associated Diseases in Organs. Oncotarget, 9(6), pp.7204–7218.
Cherng, S.T., Tam, J., Christine, P.J., & Meza, R., 2016. Modeling the Effects of E-cigarettes on Smoking Behavior. Epidemiology, 27(6), pp.819–826.
Chilosi, M., Poletti, V., & Rossi, A., 2012. The Pathogenesis of COPD and IPF: Distinct Horns of the Same Devil? Respiratory Research, 13(1), pp.3.
de-Oliveira, S., Reyes-Aldasoro, C.C., Candel, S., Renshaw, S.A., Mulero, V., & Calado, Â., 2013. Cxcl8 (IL-8) Mediates Neutrophil Recruitment and Behavior in the Zebrafish Inflammatory Response. The Journal of Immunology, 190(8), pp.4349–4359.
Dellinger, B., Khachatryan, L., Masko, S., & Lomnicki, S., 2011. Free Radicals in Tobacco Smoke. Mini-Reviews in Organic Chemistry, 8(4), pp.427–433.
Deshmukh, S., Dive, A., Moharil, R., & Munde, P., 2016. Enigmatic Insight Into Collagen. Journal of Oral and Maxillofacial Pathology, 20(2), pp.276.
Goel, R., Durand, E., Trushin, N., Prokopczyk, B., Foulds, J., Elias, R.J., & Richie, J.P., 2015. Highly Reactive Free Radicals in Electronic Cigarette Aerosols. Chemical Research in Toxicology, 28(9), pp.1675–1677.
Grana, R., Benowitz, N., & Glantz, S.A., 2014. E-Cigarettes. Circulation, 129(19), pp.1972–1986.
Herlina, N., Riyanto, S., Martono, S., & Rohman, A., 2018. Antioxidant Activities, Phenolic and Flavonoid Contents of Methanolic Extract of Stelechocarpus burahol Fruit and its Fractions. Dhaka University Journal of Pharmaceutical Sciences, 17(2), pp.153–159.
Jaber, R.M., Mirbolouk, M., DeFilippis, A.P., Maziak, W., Keith, R., Payne, T., Stokes , A., Benjamin, E., Bhatnagar, A., Blankstein, A., Saxena, A., Blaha, M.J., & Nasir, K., 2018. Electronic Cigarette Use Prevalence, Associated Factors, and Pattern by Cigarette Smoking Status in the United States From NHANES (National Health and Nutrition Examination Survey) 2013–2014. Journal of the American Heart Association, 7(14).
Jensen, R.P., Luo, W., Pankow, J.F., Strongin, R.M., & Peyton, D.H., 2015. Hidden Formaldehyde in E-Cigarette Aerosols. New England Journal of Medicine, 372(4), pp.392–394.
Jongenelis, M.I., Kameron, C., Rudaizky, D., & Pettigrew, S., 2019. Support for E-cigarette Regulations Among Australian Young Adults. BMC Public Health, 19(1), pp.67.
Karmaker, N., Lira, D.N., Das, B.K., Kumar, U., & Rouf, A.S.S., 2017. Synthesis and Antioxidant Activity of Some Novel Benzimidazole Derivatives. Dhaka University Journal of Pharmaceutical Sciences, 16(2), pp.245–249.
Levy, B.D., & Serhan, C.N., 2014. Resolution of Acute Inflammation in the Lung. Annual Review of Physiology, 76(1), pp.467–492.
Marrocco, I., Altieri, F., & Peluso, I., 2017. Measurement and Clinical Significance of Biomarkers of Oxidative Stress in Humans. Oxidative Medicine and Cellular Longevity, 2017, pp.1–32.
Martínez-Sánchez, J.M., Fu, M., Martín-Sánchez, J.C., Ballbè, M., Saltó, E., & Fernández, E., 2015. Perception of Electronic Cigarettes in the General Population: Does Their Usefulness Outweigh Their Risks? BMJ Open, 5(11), e009218.
McKleroy, W., Lee, T.-H., & Atabai, K., 2013. Always Cleave Up Your Mess: Targeting Collagen Degradation to Treat Tissue Fibrosis. American Journal of Physiology-Lung Cellular and Molecular Physiology, 304(11), pp.L709–L721.
Palipudi, K.M., Mbulo, L., Morton, J., Mbulo, L., Bunnell, R., Blutcher-Nelson, G., Asma, S., 2016. Awareness and Current Use of Electronic Cigarettes in Indonesia, Malaysia, Qatar, and Greece: Findings From 2011–2013 Global Adult Tobacco Surveys. Nicotine & Tobacco Research, 18(4), pp.501–507.
Pardo, A., Cabrera, S., Maldonado, M., & Selman, M., 2016. Role of Matrix Metalloproteinases in the Pathogenesis of Idiopathic Pulmonary Fibrosis. Respiratory Research, 17(1), pp.23.
Polosa, R., Caponnetto, P., Morjaria, J.B., Papale, G., Campagna, D., & Russo, C., 2011. Effect of an Electronic Nicotine Delivery Device (e-Cigarette ) on Smoking Reduction and Cessation : A Prospective 6-Month Pilot Study. BMC Public Health, 11.
Pratiwi, S.R., Lorensia, A., & Suryadinata, R.V., 2018. Vitamin C and E Intake with SQ-FFQ towards Smokers’ and Non-Smokers’ Lung Function. Media Kesehatan Masyarakat Indonesia, 14(2), pp.101.
Rathnayake, N., Gieselmann, D.-R., Heikkinen, A., Tervahartiala, T., & Sorsa, T., 2017. Salivary Diagnostics—Point-of-Care Diagnostics of MMP-8 in Dentistry and Medicine. Diagnostics, 7(1), pp.7.
Ravipati, A.S., Zhang, L., Koyyalamudi, S.R., Jeong, S.C., Reddy, N., Bartlett, J., Smith, P.T., Shanmugam, K., Münch, G., Wu, M.J., Satyanarayanan, M., & Vysetti, B., 2012. Antioxidant and Anti-inflammatory Activities of Selected Chinese Medicinal Plants and their Relation with Antioxidant Content. BMC Complementary and Alternative Medicine, 12(1), pp.1192.
Reuter, S., Gupta, S.C., Chaturvedi, M.M., & Aggarwal, B.B., 2010. Oxidative Stress, Inflammation, and Cancer: How are They Linked? Free Radical Biology and Medicine, 49(11), pp.1603–1616.
Sagor, M.A.T., Reza, H.M., Tabassum, N., Rahman, M.M., & Alam, M.A., 2017. Fresh Bitter Melon Fruit (Momordica charantia) Attenuated Oxidative Stress, Fibrosis and Renal Injury in Carbon Tetrachloride Treated Rats. Dhaka University Journal of Pharmaceutical Sciences, 16(2), pp.205–214.
Suryadinata, R.V., Wirjatmadi, B., & Adriani, M., 2016. Pengaruh Perubahan Hiperplasia Sel Goblet Selama 28 Hari Paparan Asap Rokok Dengan Pemberian Antioksidan Superoxide Dismutase. The Indonesian Journal of Public Health, 11(1), pp.60.
Suryadinata, R.V., Wirjatmadi, B., & Adriani, M., 2017. Effectiveness Decrease Combined with Supplements Malondialdehyde Antioxidant Superoxide Dismutase Gliadin Melon with Due to Exposure to Cigarette. Global Medical And Health Communication, 5(2), pp.79–83.
Suryadinata, R.V., Lorensia, A., & Sari, R.K., 2017. Differences in Nutrition Food Intake and Body Mass Index between Smoker and Non-smoker in Adult. Indonesian Journal of Clinical Pharmacy, 6(3), pp.171–180.
Suryadinata, R.V. & Wirjatmadi, B., 2020. Selenium Linked to Increased Antioxidant Levels and Decreased Free Radicals in Lung Tissue of Wistar Rats Exposed to E-Cigarette Smoke. Journal of Global Pharma Technology, 12(9), pp.32-39
Thirkettle, S., Decock, J., Arnold, H., Pennington, C.J., Jaworski, D.M., & Edwards, D.R., 2013. Matrix Metalloproteinase 8 (Collagenase 2) Induces the Expression of Interleukins 6 and 8 in Breast Cancer Cells. Journal of Biological Chemistry, 288(23), pp.16282–16294.
Thrasher, J.F., Abad-Vivero, E.N., Barrientos-Gutíerrez, I., Pérez-Hernández, R., Reynales-Shigematsu, L.M., Mejía, R., & Sargent, J.D., 2016. Prevalence and Correlates of E-Cigarette Perceptions and Trial Among Early Adolescents in Mexico. Journal of Adolescent Health, 58(3), pp.358–365.
Vardavas, C.I., Filippidis, F.T., & Agaku, I.T., 2015. Determinants and Prevalence of E-cigarette Use Throughout the European Union: A Secondary Analysis of 26 566 Youth and Adults from 27 Countries. Tobacco Control, 24(5), pp.442–448.
Virlando, S.R., 2018. Effect of Free Radicals on Inflammatory Process in Chronic Obstructive Pulmonary Disease (COPD). Amerta Nutrition, 2(4), pp.317–324.
Voigt, K., 2015. Smoking Norms and the Regulation of E-Cigarettes. American Journal of Public Health, 105(10), pp.1967–1972.
Wieczorek, M., Abualrous, E.T., Sticht, J., Álvaro-Benito, M., Stolzenberg, S., Noé, F., & Freund, C., 2017. Major Histocompatibility Complex (MHC) Class I and MHC Class II Proteins: Conformational Plasticity in Antigen Presentation. Frontiers in Immunology, 8.
Wirjatmadi, B., & Suryadinata, R.V., 2020. The Alteration on Malondialdehyde Content on Wistar Rats’ Blood and Lungs Tissue to Ward the Exposure of Electric Cigarette Smoke. Indian Journal of Public Health Research & Development, 11(3), pp.1881-1887.
Wojdasiewicz, P., Poniatowski, Ł.A., & Szukiewicz, D., 2014. The Role of Inflammatory and Anti-Inflammatory Cytokines in the Pathogenesis of Osteoarthritis. Mediators of Inflammation, 2014, pp.1–19.
Xu, Y., Guo, Y., Liu, K., Liu, Z., & Wang, X., 2016. E-Cigarette Awareness, Use, and Harm Perception among Adults: A Meta-Analysis of Observational Studies. Plos One, 11(11), pp.e0165938.
Zhang, G., Wang, Z., Zhang, K., Hou, R., Xing, C., Yu, Q., & Liu, E., 2018. Safety Assessment of Electronic Cigarettes and Their Relationship with Cardiovascular Disease. International Journal of Environmental Research and Public Health, 15(1), pp.75.
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