Chemical Composition and In vitro Antibacterial and Cytotoxic Effect of Nigella sativa L. Seed Extract

Callixte Cyuzuzo(1), Heny Arwati(2), Tuyishimire Irene(3), Shahzad Shoukat(4),

DOI: https://doi.org/10.15294/kemas.v16i3.26799


(1) Postgraduate Program in Immunology, Postgraduate School, Universitas Airlangga, Surabaya, Indonesia
(2) Postgraduate Program in Immunology, Postgraduate School, Universitas Airlangga, Surabaya, Indonesia
(3) Department of Horticulture, College of Agriculture, Animal Science and Veterinary Medicine, University of Rwanda, Rwanda
(4) Department of Horticulture, College of Agriculture, Animal Science and Veterinary Medicine, University of Rwanda, Rwanda

Abstract

Nigella sativa L. is a plant with high medicinal profile in the treatment of diseases. This study aimed to assess the chemical composition and in vitro antibacterial and cytotoxic effect of Nigella sativa L. seed extract. Seeds were purchased from supermarket and blended into powder using electric blender. The powder was macerated with 96% methanol. The extracts were then qualitatively screened to test the presence of secondary metabolites and agar well diffusion method was employed to study the antibacterial activity of extracts. In this evaluation, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to examine the cytotoxic effect of extract on peripheral blood mononuclear cells (PBMCs). MTT assay was done on different extract concentrations for 3 days and the results were recorded daily. The findings revealed the presence of tannins, terpenoids, steroids, saponins, alkaloids, phenolic compounds and flavonoids. The antibacterial activity was observed with the inhibition zones ranging from 11.3 ±1.1 to 16.3 ±1.5mm while the minimum inhibitory concentration (MIC) values of extract ranged between 0.5 and 1.25 μg/mL. The black seed extracts contain bioactive substances with germicidal activity and in vitro administration of black seed extract up to the dose of 100 μg/mL resulted no cytotoxic effect on PBMCs.

Keywords

antibacterial, cytotoxicity, Nigella sativa L, MIC, inhibition zone

Full Text:

PDF

References

N-Hexane Extract of Nigella Sativa against Some Pathogenic Bacteria. American Journal of Biomedical Science & Research, 6(5), pp.430–434.

Ahmad, A., Husain, A., Mujeeb, M., Khan, S.A., Najmi, A.K., Siddique, N.A., Damanhouri, Z.A., & Anwar, F., 2013. A Review on Therapeutic Potential of Nigella sativa: A Miracle Herb. Asian Pacific Journal of Tropical Biomedicine, 3(5), pp.337–352.

Al-Homidan, A., Al-Qarawi, A.A., Al-Waily, S.A., & Adam, S.E.I., 2002. Response of Broiler Chicks to Dietary Rhazya stricta and Nigella sativa. British Poultry Science, 43(2), pp.291–296.

Aljabre, S.H.M., Alakloby, O.M., & Randhawa, M.A., 2015. Dermatological Effects of Nigella sativa. Journal of Dermatology & Dermatologic Surgery, 19(2), pp.92–98.

Amin, B., & Hosseinzadeh, H., 2016. Black Cumin (Nigella sativa) and Its Active Constituent, Thymoquinone: An Overview on the Analgesic and Anti-inflammatory Effects. Planta Medica, 82(1–2), pp.8–16.

Azizi, S., Shahri, M.M., Rahman, H.S., Rahim, R.A., Rasedee, A., & Mohamad, R., 2017. Green Synthesis Palladium Nanoparticles Mediated by White Tea (Camellia sinensis) Extract with Antioxidant, Antibacterial, and Antiproliferative Activities Toward the Human Leukemia (MOLT-4) Cell Line. International Journal of Nanomedicine, 12, pp.8841–8853.

Callixte, C., & Dusabimana, J.D., Ma’ruf, A., Dachlan, Y.P., Sensusiati, A.D., Daniel, N.W.E., 2020. Phytoconstituent Analysis and Antibacterial Potential of Epicarp Extracts from Mature Fruits of Persea americana Mill. Molecular and Cellular Biomedical Sciences, 4(2), pp.94–99.

Callixte, C., Baptiste, N.J., & Arwati, H., 2020. Phytochemical Screening and Antimicrobial Activities of Methanolic and Aqueous Leaf Extracts of Carica papaya Grown in Rwanda. Molecular and Cellular Biomedical Sciences, 4(1), pp.39.

Dollah, M.A., Parhizkar, S., Latiff, L.A., & Hassan, M.H.B., 2013. Toxicity Effect of Nigella sativa on the Liver Function of Rats. Advanced Pharmaceutical Bulletin, 3(1), pp.97–102.

El-Naggar, T., Gómez-Serranillos, M. P., Palomino, O. M., Arce, C., & Carretero, M.E., 2010. Nigella sativa L. Seed Extract Modulates the Neurotransmitter Amino Acids Release in Cultured Neurons in Vitro. Journal of Biomedicine and Biotechnology, 2010.

Gracelin, D.H.S., Britto, J., De-eya, P.B., & Kumar, R., 2012. Antibacterial Screening Of A Few Medicinal Ferns Against Antibiotic Resistant Phyto Pathogen. International Journal of Pharmaceutical Sciences and Research, 3(03), pp.868–873.

Hamza, R.Z., & Al-Harbi, M.S., 2015. Amelioration of Paracetamol Hepatotoxicity and Oxidative Stress on Mice Liver with Silymarin and Nigella sativa Extract Supplements. Asian Pacific Journal of Tropical Biomedicine, 5(7), pp.521–531.

Hasan, N.A., Nawahwi, M.Z., & Malek, H.A., 2013. Antimicrobial Activity of Nigella sativa Seed Extract. Sains Malaysiana, 42(2), pp.143–147.

Hassan, M.Q., Akhtar, M., Ahmed, S., Ahmad, A., & Najmi, A.K., 2017. Nigella sativa Protects Against Isoproterenol-induced Myocardial Infarction by Alleviating Oxidative Stress, Biochemical Alterations and Histological Damage. Asian Pacific Journal of Tropical Biomedicine, 7(4), pp.294–299.

Islam, M.H., Ahmad, I.Z., & Salman, M.T., 2012. Antibacterial Activity of Nigella sativa Seed in Various Germination Phases on Clinical Bacterial Strains Isolated from Human Patients. E3 Journal of Biotechnology and Pharmaceutical Research, 4, pp.8–13.

Kaleena, P.K., Philip, D., Valivittan, K., & Girish Kumar, C.P., 2011. Phytochemical Screening and Antimicrobial Activity of Sansevieria roxburghiana Schult. and Schult. F. Middle-East Journal of Scientific Research, 10(4), pp.512–518.

Khan, M.A., Aldebasi, Y.H., Alsuhaibani, S.A., AlSahli, M.A., Alzohairy, M.A., Khan, A., & Younus, H., 2018. Therapeutic Potential of Thymoquinone Liposomes Against the Systemic Infection of Candida albicans in Diabetic Mice. PLoS ONE, 13(12), pp.1–17.

Khan, M.A., 2019. Thymoquinone, A Constituent of Prophetic Medicine-black Seed, is a Miracle Therapeutic Molecule Against Multiple Diseases. International Journal of Health Sciences, 13(1), pp.1–2.

Kiari, F.Z., Meddah, B., & Tir, T.M.A., 2018. In Vitro Study on the Activity of Essential Oil and Methanolic Extract from Algerian Nigella sativa L. Seeds on the Growth Kinetics of Micro-Organisms Isolated from the Buccal Cavities of Periodontal Patients. Saudi Dental Journal, 30(4), pp.312–323.

Kumar, A., & Rehman, H., 2019. Antibacterial, Antioxidant, Cytotoxicity and Qualitative Phyto- Chemical Evaluation of Seed Extracts of Nigella sativa and Its Introduction.

Mahmoud, S.S., & Torchilin, V.P., 2013. Hormetic/Cytotoxic Effects of Nigella sativa Seed Alcoholic and Aqueous Extracts on MCF-7 Breast Cancer Cells Alone or in Combination with Doxorubicin. Cell Biochemistry and Biophysics, 66(3), pp.451–460.

Mansour, M.A., Ginawi, O.T., El-Hadiyah, T., El-Khatib, A.S., Al-Shabanah, O.A., & Al-Sawaf, H.A., 2001. Effects of Volatile Oil Constituents of Nigella sativa on Carbon Tetrachloride-Induced Hepatotoxicity in Mice: Evidence for Antioxidant Effects of Thymoquinone. Research Communications in Molecular Pathology and Pharmacology, 110(3–4), pp.239–251.

Mohammed, S.J., Amin, H.H.H., Aziz, S.B., Sha, A.M., Hassan, S., Abdul Aziz, J.M., & Rahman, H.S., 2019. Structural Characterization, Antimicrobial Activity, and in Vitro Cytotoxicity Effect of Black Seed Oil. Evidence-Based Complementary and Alternative Medicine, 2019.

Nassima, B., Nassima, B., & Riadh, K., 2019. Antimicrobial and Antibiofilm Activities of Phenolic Compounds Extracted from Populus nigra and Populus Alba Buds (Algeria). Brazilian Journal of Pharmaceutical Sciences, 55.

Nehar, S., Rani, P., Kumar, C., Kauser, H., & Alam, I., 2015. Evaluation on Cytotoxicity of Ethanolic Extract of Nigella sativa Seed. American Journal of Ethnomedicine, 2(5), pp.276–283.

Paarakh, P.M., 2010. Nigella sativa Linn.- A Comprehensive Review. Indian Journal of Natural Products and Resources, 1(4), pp.409–429.

Paz, M., Gúllon, P., Barroso, M.F., Carvalho, A.P., Domingues, V.F., Gomes, A.M., Becker, H., Longhinotti, E., & Delerue-Matos, C., 2015. Brazilian Fruit Pulps as Functional Foods and Additives: Evaluation of Bioactive Compounds. Food Chemistry, 172, pp.462–468.

Rahman, H.S., Rasedee, A., Abdul, A.B., Zeenathul, N.A., Othman, H.H., Yeap, S.K., How, C.W., & Wan, N.H., W, A.G., 2014. Zerumbone-loaded Nanostructured Lipid Carrier Induces G2/M Cell Cycle Arrest and Apoptosis Via Mitochondrial Pathway in a Human Lymphoblastic Leukemia Cell Line. International Journal of Nanomedicine, 9(1), pp.527–538.

Scandorieiro, S., de Camargo, L.C., Lancheros, C.A.C., Yamada-Ogatta, S.F., Nakamura, C.V., de Oliveira, A.G., Andrade, C.G.T.J., Duran, N., Nakazato, G., & Kobayashi, R.K.T., 2016. Synergistic and Additive Effect of Oregano Essential Oil and Biological Silver Nanoparticles Against Multidrug-resistant Bacterial Strains. Frontiers in Microbiology, 7, pp.1–14.

Soni, A., & Sosa, S., 2013. Phytochemical Analysis and Free Radical Scavenging Potential of Herbal and Medicinal Plant Extracts. Journal of Pharmacognosy and Phytochemistry JPP, 22(24), pp.22–29.

Swamy, S.M.K., & Tan, B.K.H., 2000. Cytotoxic and Immunopotentiating Effects of Ethanolic Extract of Nigella sativa L. seeds. Journal of Ethnopharmacology, 70(1), pp.1–7.

Ugur, A. R., Dagi, H.T., Ozturk, B., Tekin, G., & Findik, D., 2016. Assessment of in Vitro Antibacterial Activity and Cytotoxicity Effect of Nigella sativa oil. Pharmacognosy Magazine, 12(47), pp.S471–S474.

Wayne, P., 2013. M100-S23 Performance Standards for Antimicrobial (Issue January).

Woo, C.C., Kumar, A.P., Sethi, G., & Tan, K.H.B., 2012. Thymoquinone: Potential Cure for Inflammatory Disorders and Cancer. Biochemical Pharmacology, 83(4), pp.443–451.

World Health Organization (WHO)., 2013. WHO Traditional Medicine Strategy 2014-2023. World Health Organization (WHO), pp.1–76.

Yildiz, F., Coban, S., Terzi, A., Savas, M., Bitiren, M., Celik, H., & Aksoy, N., 2010. Protective Effects of Nigella Sativa Against Ischemia-reperfusion Injury of Kidneys. Renal Failure, 32(1), pp.126–131.

Younus, H., 2018. Younus, H., 2018. Molecular and Therapeutic actions of Thymoquinone Actions of Thymoquinone, Livre. (I. Younus, Hina (Interdisciplinary Biotechnology Unit Aligarh Muslim University Aligarh, Uttar Pradesh (ed.); 1st ed.). Berlin, Germany ,Springer US.

Zaoui, A., Cherrah, Y., Alaoui, K., Mahassine, N., Amarouch, H., & Hassar, M., 2002. Effects of Nigella sativa Fixed Oil on Blood Homeostasis in Rat. Journal of Ethnopharmacology, 79(1), pp.23–26.

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.