Transovarial Infection of Dengue Virus in Aedes aegypti and Aedes albopictus

Wanti Wanti(1), Isnawati Isnawati(2), Titik Respati(3),

(1) Pusat Unggulan IPTEKS Pengendalian Penyakit Tropis Berbasis Kepulauan Poltekkes Kemenkes Kupang
(2) Poltekkes Kemenkes Banjarmasin
(3) Faculty of Medicine, Universitas Islam Bandung


Nowadays, Dengue Haemorrhagic Fever (DHF) is still a significant health problem. Including in Kupang City. The existence of transovarial infection of Dengue virus is essential to know to support the prevention to be more effective and efficient. This study aimed to determine the existence of transovarial infectious and the serotypes variability of Dengue virus in Aedes sp in Kupang City. This observational research took place in nine villages in Kupang City in 2017. Where 20 houses in each village are observed to collect Aedes sp eggs. Cluster sampling was conducted to choose houses with DHF cases and surrounding areas 100 m from the cases. Ovitrap and Ovistrip were used to collect Aedes sp eggs, then Aedes sp eggs are reared in Parasitology Laboratory UGM. Adults mosquitoes from rearing were observed the transovarial Infection and Dengue Virus Serotype by One Step RT-PCR followed by nested PCR. This research found a transovarial infection in Aedes aegypti  and Aedes albopictus   and for Denvir-2 and Denvir-3. This finding is a change from previous findings in the same place where only Denvir-1 was transmitted transovarially in Aedes sp.


Aedes sp, Dengue, Infection, Transovarial, Serotypes

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Ali, K.M., Asha, A.V., & Aneesh, E.M., 2014. Bioecology and Vectorial Capacity of Aedes Mosquitoes (Diptera: Culicidae) in Irinjalakuda Municipality, Kerala, India in Relation to Disease Transmission. International Journal of Current Research and Academic Review, 2(4), pp.43–49.

Angel, B., & Joshi, V., 2008. Distribution and Seasonality of Vertically Transmitted Dengue Viruses in Aedes Mosquitoes in Arid and Semi-Arid Areas of Rajasthan, India. J Vector Borne Dis, 45, pp.56–59.

Cecílio, S.G., Junior, W.F.S., Totola, A.H., Magalhaes, C.L.D.B., Ferreira, J.M.S., & Magalhaes, J.C.D., 2015. Dengue Virus Detection in Aedes aegypti Larvae from Southeastern Brazil. J Vector Ecology, 40(1), pp.71–74.

Chen, W.J., Wei, H.L., Hsu, E.L., & Chen, E.R., 1993. Vector Competence of Aedes albopictus and Ae. aegypti (Diptera: Culicidae) to Dengue 1 Virus on Taiwan: Development of the Virus in orally and Parenterally Infected Mosquitoes. J Med Entomol, 30(3), pp.524–30.

Chew, M.H., Rahman, M., & Hussin, S., 2015. Molecular Epidemiology and Phylogenetic Analysis of Dengue Virus Type-1 and 2 Isolated in Malaysia. Pakistan Journal of Medical Sciences, 31(3), pp.615–620.

Deng, S.-Q., Yang, X., Wei, Y., Chen, J.-T., Wang, X.-J., & Peng, H.-J., 2020. A Review on Dengue Vaccine Development. Vaccines, 8(1), pp.63.

Esteva, L., & Vargas, C., 2000. Influence of Vertical and Mechanical Transmission on the Dynamics of Dengue Disease. Math Biosci, 167, pp.51–64.

Ferreira-de-Lima, V.H., & Lima-Camara, T.N., 2018. Natural Vertical Transmission of Dengue Virus in Aedes aegypti and Aedes albopictus: a Systematic Review. Parasites & Vectors, 11(1), pp.77.

Harapan, H., Michie, A., Mudatsir, M., Sasmono, R.T., & Imrie, A., 2019. Epidemiology of Dengue Hemorrhagic Fever in Indonesia : Analysis of Five Decades Data from the National Disease Surveillance. BMC Research Notes, 12.

Kemenkes RI., 2019. Profil Kesehatan Indonesia 2018. Jakarta: Kemenkes RI.

Lanciotti, R.S., Calisher, C.H., Gubler, D.J., Chang, G., & Vorndamt, A.V., 1992. Rapid Detection and Typing of Dengue Viruses from Clinical Samples by Using Reverse Transcriptase-Polymerase Chain Reaction. J Clin Microbiol, 30(3), pp.545–551.

Martín, J.L.S., Brathwaite, O., Zambrano, B., Solórzano, J.O., Bouckenooghe, A., Dayan, G.H., & Guzmán, M.G., 2010. The Epidemiology of Dengue in the Americas Over the Last Three Decades: A Worrisome Reality. Am J Trop Med Hyg, 82(1), pp.128–135.

Martínez, N.E., Dzul-Manzanilla, F., Gutiérrez-Castro, C., Ibarra-López, J., Bibiano-Marín, W., López-Damián, L., & Martini-Jaimes, A., 2014. Natural Vertical Transmission of Dengue-1 Virus in Aedes aegypti Populations in Acapulco, Mexico. Journal of the American Mosquito Control Association, 30(2), pp.143–146.

Medical Research Institute & Dengue Coordination Unit., 2011. Practical Manual and Gudelines for Dengue Vector Surveilance. Sri Lanka: Medical Research Institute & Dengue Coordination Unit.

Murray, N.E.A., Quam, M.B., & Wilder-Smith, A., 2013. Epidemiology of Dengue Past Present and Future Prospects. Clinical Epidemiology, 3(5), pp.299–309.

Poole-Smith, B.K., Hemme, R.R., Delorey, M., Felix, G., Gonzalez, A.L., Amador, M., & Hunsperger, E.A., 2015. Comparison of Vector Competence of Aedes Mediovittatus and Aedes aegypti for Dengue Virus: Implications for Dengue Control in the Caribbean. PLOS Neglected Tropical Diseases, 9(2), pp.e0003462.

Putri, D.F., Widya, A., Sugeng, M.J., & Sitti, U.R., 2018. The Potency of Polygamy Behavior in Aedes aegypti Mosquitoes by Venereal Transmission Dengue Virus. KEMAS: Jurnal Kesehatan Masyarakatkat, 13(3), pp.382–388.

Richards, S.L., Anderson, S.L., & Alto, B.W., 2012. Vector Competence of Aedes aegypti and Aedes albopictus (Diptera: Culicidae) for Dengue Virus in the Florida Keys. Journal of Medical Entomology, 49(4), pp.942–6.

Rohani, A., Azahary, A.R.A., Malinda, M., Zurainee, M.N., Rozilawati, H., Najdah, W.M.A.W., & Lee, H.L., 2014. Eco-Virological Survey of Aedes Mosquito Larvae in Selected Dengue Outbreak Areas in Malaysia. J Vector Borne Dis, 51, pp.327–332.

Thongrungkiat, S., Maneekan, P., Wasinpiyamongkol, L., & Prummongkol, S., 2011. Prospective Field Study of Transovarial Dengue-virus Transmission by two Different Forms of Aedes aegypti in an Urban area of Bangkok, Thailand. Journal of Vector Ecology, 36(1), pp.147–152.

Usman, H., & Akbar, P.S., 2009. Introduction to Statistics. Jakarta: Bumi Aksara.

Vikram, K., Nagpal, B.N., Pande, V., Srivastava, A., Saxena, R., Singh, H., & Anushrita., 2015. Detection of Dengue Virus in Individual Aedes aegypti Mosquitoes in Delhi, India. J Vector Borne Dis, 52, pp.129–133.

Wanti, W., Sila, O., Irfan, I., & Sinaga, E., 2016. Transovarial Transmission and Dengue Virus Serotypes in Aedes aegypti in Kupang. KEMAS: Jurnal Kesehatan Masyarakat, 12(1), pp.131–138.

Wanti, W., Yudhastuti, R., Notobroto, H.B., Subekti, S., Agustina, A., & Ekawati, C., 2019. Container Characteristics and Dengue Hemorrhagic Fever Incidence. International Journal of Public Health Science (IJPHS), 8(3), pp.314.

Wanti., Yudhastuti, R., Yotopranoto, S., Notobroto, H.B., Subekti, S., & Umniati, S.R., 2017. Container Positivity and Larva Distribution Based on the Container Characteristic. International Journal of Public Health Science (IJPHS), 6(3), pp.237–242.

Yan, H., Ding, Z., Yan, J., Yao, W., Pan, J., Yang, Z., & Lou, X., 2018. Epidemiological Characterization of the 2017 Dengue Outbreak in Zhejiang, China and Molecular Characterization of the Viruses. Frontiers in Cellular and Infection Microbiology, 8

Yung, C.-F., Lee, K.-S., Thein, T.-L., Tan, L.-K., Gan, V.C., Wong, J.G.X., & Lye, D.C., 2015. Dengue Serotype-Specific Differences in Clinical Manifestation, Laboratory Parameters and Risk of Severe Disease in Adults, Singapore. The American Journal of Tropical Medicine and Hygiene, 92(5), pp.999–1005.


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