PENINGKATAN CD8 DAN IFN-γ PADA HYPERSENSITIVITY PNEUMONITIS (HP) AKIBAT PAJANAN DEBU PENGGILINGAN PADI

##plugins.themes.academic_pro.article.main##

Isa Ma'rufi
Kuntoro Kuntoro
Soejdajadi Keman

Abstract

Abstrak


 


 


Masalah klinis kesehatan kerja pada pekerja penggilingan padi saat ini adalah penyakit saluran pernafasan. Penelitian ini bertujuan untuk menganalisis peningkatan CD8 dan IFN-γ pada Pneumonitis Hipersensitif (HP) akibat pajanan debu penggilingan padi pada mencit  (Musmusculus) BALB/C. Penelitian yang dilakukan adalah penelitian eksperimental laboratoris dengan hewan coba mencit (Musmusculus) BALB/C,  rancangan penelitian yang digunakan adalah Randomized the post test only control group design. Mencit  (Musmusculus) BALB/C dipajan dengan debu penggilingan padi selama empat jam/hari selama tiga puluh hari dengan konsentrasi 0.50 mg/m3, 0.75 mg/m3, 1.00 mg/m3. Variabel bebas adalah debu penggilingan padi, sedangkan variabel tergantung adalah   CD8 dan IFN-γ, serta gambaran histopatologis paru mencit, sedangkan variabel kendali adalah strain, berat badan, dan umur. Hasil penelitian menunjukkan bahwa terjadi peningkatan kadar CD8 antara kontrol dengan perlakuan, dan secara statistik  ada perbedaan yang bermakna. Kemudian terjadi peningkatan IFN-γ dan secara statistik ada perbedaan yang bermakna antara kontrol dan perlakuan pada mencit  (Musmusculus) BALB/C. Hasil penelitian dapat digunakan sebagai dasar upaya pencegahan dan pemberian terapi penyakit HP lebih dini sehingga dapat meningkatkan kualitas paru.


 


Kata Kunci: CD8, IFN-γ,  debu penggilingan padi


 


 


Abtract


 


The purpose of this research was to analyze increase of CD8  and IFN-γ of Hypersensitivity Pneumonitis (HP) as a result from the exposure of dust from paddy milling on mice (Musmusculus)BALB/C. The research done was a laboratory experimental research with mice (MusMusculus) as experimental animal.  The research design used was the post only control group design using mice (MusMusculus) Balb/c as experimental animal.  Mice (Musmusculus) Balb/c were exposed to dust from paddy milling for four (4) hours/day and it was done for thirty (30) days with the exposed concentrations respectively were 0.50 mg/m3, 0.75 mg/m3, 1.00 mg/m3.The research variables were free variable, dependent variable, and control variable.  Independent variable was dust from paddy milling, dependent variables were Hypersensitivity Pneumonitis (HP), CD8 and IFN-γ, while control variables were strain, body weight and age of mice (MusMusculus) Balb/c. The research result showed that there was an increase of CD8, and statistically there was significant difference; there was an increase on IFN-γ and statistically there was a significant difference between the study and control on mice BALB/C. It was suggested that:  the research result be used to underlie a preventive action and an early therapy on Hypersensitivity Pneumonitis (HP) so that it increase the quality of lung.


 


Keywords: CD8, IFN-γ, dust from paddy milling


 


 


Abtract


 


The purpose of this research was to analyze increase of CD8  and IFN-γ of Hypersensitivity Pneumonitis (HP) as a result from the exposure of dust from paddy milling on mice (Musmusculus)BALB/C. The research done was a laboratory experimental research with mice (MusMusculus) as experimental animal.  The research design used was the post only control group design using mice (MusMusculus) Balb/c as experimental animal.  Mice (Musmusculus) Balb/c were exposed to dust from paddy milling for four (4) hours/day and it was done for thirty (30) days with the exposed concentrations respectively were 0.50 mg/m3, 0.75 mg/m3, 1.00 mg/m3.The research variables were free variable, dependent variable, and control variable.  Independent variable was dust from paddy milling, dependent variables were Hypersensitivity Pneumonitis (HP), CD8 and IFN-γ, while control variables were strain, body weight and age of mice (MusMusculus) Balb/c. The research result showed that there was an increase of CD8, and statistically there was significant difference; there was an increase on IFN-γ and statistically there was a significant difference between the study and control on mice BALB/C. It was suggested that:  the research result be used to underlie a preventive action and an early therapy on Hypersensitivity Pneumonitis (HP) so that it increase the quality of lung.


 


Keywords: CD8, IFN-γ, dust from paddy milling


 

##plugins.themes.academic_pro.article.details##

How to Cite
Ma’rufi, I., Kuntoro, K., & Keman, S. (2017). PENINGKATAN CD8 DAN IFN-γ PADA HYPERSENSITIVITY PNEUMONITIS (HP) AKIBAT PAJANAN DEBU PENGGILINGAN PADI. Unnes Journal of Public Health, 6(4), 253-259. https://doi.org/10.15294/ujph.v6i4.17732

References

Abbas, A.K., Lichtman, A.H. 2009. Basic immunology: Functions and disorders of the immune system, 3nd edition. Philadelphia: Saunders Elsevier

Ando, M., Konishi, K., Yoneda, R., Tamura, M. 1991. Difference in the phenotypes of bronchoalveolar lavage lymphocytes in patient with summer-type hypersensitivity pneumonitis, farmer’s Lung, ventilation pneumonitis, and bird fancier’s lung: report of a nationwide epidemiologic study in Japan. J Allergy Clin Immunol, 87: 1002-1009

Banham, S.W., McSharry, C., Lynch, P.P., Boyd, G. 1986. Relationships between avian exposure, humoral immune response, and pigeon breeder’s disease among Scottish pigeon fanciers. Thorax, 41: 274-278

Bantacut, T. 2006. Peningkatan Daya Saing Beras Melalui Perbaikan Kualitas. Makalah ini disampaikan pada Lokakarya Nasional Gedung Pertemuan Oryza Bulog. Jakarta, 13 September 2006

Barrera, L., Mendoza, F., Zuñiga, J., Estrada, A., Zamora, A. C., Melendro, E. I., & Selman, M. 2008. Functional diversity of T-cell subpopulations in subacute and chronic hypersensitivity pneumonitis. American journal of respiratory and critical care medicine, 177 (1): 44-55

Bourke, S.J., Dalphin, J.C., Boyd, G., McSharry, C., Baldwin, C.I., Calvert, J.E., 2001. Hypersensitivity pneumonitis: Current concepts. Eur Respir J, 18: Suppl. 32: 81s–92s.

Cormier, Y., Schuyler, M. 2006. Hypersensitivity pneumonitis and organic dust toxic syndromes. In Bernstein L (ed). Asthma in the workplace, 3nd edition. New York: Taylor & Francis Group

Denis, M., Ghadirian, E. 1992. Murine hypersensitivity pneumonitis: Bidirectional role of interferon-gamma. Clin Exp Allergy, 8:783-92

Facco, M., Trentin, L., Nicolardi. 2004. T cells in the lung of patients with hypersensitivity pneumonitis accumulate in a clonal manner. J Leukoc Biol, 75: 798-804

Farber, H.J., Varghese, N.S.P., 2009. Hypersensitivity Pneumonitis. eMedicine, Specialties>Pediatrics:GeneralMedicine>Pulmonology/299174-overview.htm

Hwang, S. J., Kim, S., Park, W. S., & Chung, D. H. 2006. IL-4-secreting NKT cells prevent hypersensitivity pneumonitis by suppressing IFN-γ-producing neutrophils. The Journal of Immunology, 177(8), 5258-5268.

Joshi, A. D., Fong, D. J., Oak, S. R., Trujillo, G., Flaherty, K. R., Martinez, F. J., & Hogaboam, C. M. 2009. Interleukin-17–mediated immunopathogenesis in experimental hypersensitivity pneumonitis. American journal of respiratory and critical care medicine, 179 (8): 705-716

Korosec, P., Osolnik, K., Kern, I., Silar, M., Mohorcic, K., Kosnik, M. 2007. Expansion of pulmonary CD8+CD56+ Natural killer T-cells in hypersensitivity pneumonitis. Chest, 132:1291-1297

Nakazawa, T., Tochigi, T. 1989. Hypersensitivity pneumonitis due to mushroom spores. Chest, 95: 1149-1151.

Nance, S., Cross, R., Yi, A. K., & Fitzpatrick, E. A. 2005. IFN‐γ production by innate immune cells is sufficient for development of hypersensitivity pneumonitis. European Journal of Immunology, 35 (6): 1928-1938

Rose, C.S. 2005. Hypersensitivity pneumonitis. In Murray and Nadel’s. Textbook of Respiratory Medicine, 4nd edition. Philadelphia: Elsevier Inc

Simonian, P. L., Roark, C. L., Wehrmann, F., Lanham, A. K., del Valle, F. D., Born, W. K., & Fontenot, A. P. 2009. Th17-polarized immune response in a murine model of hypersensitivity pneumonitis and lung fibrosis. The Journal of Immunology, 182 (1): 657-665

Trentin, L., Zambello, R., Facco, M. 1997. Selection of T lymphocytes bearing limited TCR-Vbeta regions in the lung of hypersensitivity pneumonitis and sarcoidosis. J Respir Crit care Med, 155: 587-596

Woda, B.A. 2008. Hypersensitivity pneumonitis: An immunopathology review. Arch Pathol Lab Med,132: 204–205.

Yamasaki, H., Ando, M., Brazer, W., Center, D. M., & Cruikshank, W. W. 1999. Polarized type 1 cytokine profile in bronchoalveolar lavage T cells of patients with hypersensitivity pneumonitis. The Journal of Immunology, 163(6): 3516-3523