Resistance Status of Aedes albopictus (Skuse) on Malathion in Bengkulu City
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Abstract
Abstract
Dengue Hemorrhagic Fever (DHF) is a disease caused by dengue virus and transmitted by Aedes aegypti mosquito as the main vector and Aedes albopictus as secondary vector. In 2016, Bengkulu City was one of 3 cities that experiencing DHF outbreaks in Indonesia. Insecticides malathion have been used since 1990 in bulk in DHF control programs in Bengkulu City and have not had an evaluation report on Aedes sp. vulnerability to malathion. The purpose of this research was to know the resistance status of Ae albopictus mosquitoes from endemic and sporadic areas of DHF in Bengkulu City to malathion. The sample of the study was the Ae. albopictus adult female mosquitoes which collected from endemic and sporadic areas of DHF in Bengkulu City with ovitrap installation. The resistance test was performed by CDC Bottle Bioassay method with malation 96% and 50 μg/ml diagnostic dose of. The results of this study showed 1% mortality on endemic area and 5% on sporadic area. Aedes albopictus of both areas were resistant to malathion.
Keywords: Ae. albopictus, bottle bioassay, malathion.
Abstrak
Demam Berdarah Dengue (DBD) merupakan penyakit yang disebabkan oleh virus dengue dan ditularkan oleh nyamuk Aedes aegypti sebagai vektor utama dan Aedes albopictus sebagai vektor sekunder. Pada tahun 2016, Kota Bengkulu merupakan 1 dari 3 kota yang mengalami KLB DBD di Indonesia. Insektisida malation telah digunakan sejak tahun 1990 secara massal dalam program pengendalian DBD di Kota Bengkulu dan belum memiliki laporan evaluasi mengenai kerentanan Aedes sp terhadap malation. Tujuan penelitian untuk mengetahui status kerentanan nyamuk Ae. albopictus dari daerah endemis dan sporadis DBD Kota Bengkulu terhadap malation. Sampel penelitian adalah nyamuk betina dewasa Ae. albopictus yang dikoleksi dari daerah endemik dan sporadis DBD Kota Bengkulu dengan pemasangan ovitrap. Uji kerentanan dilakukan dengan metode CDC Bottle Bioassay dengan malation 96% dan dosis diagnostik 50 µg/ml. Hasil penelitian ini menunjukkan mortalitas 1% untuk daerah endemis dan 5% untuk daerah sporadis. Aedes albopictus kedua daerah telah resisten terhadap malation.
Kata Kunci: Ae. albopictus, bottle bioassay, malation.
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References
CDC. 2010. Guideline for Evaluating Insecticide Resistance in Vectors Using the CDC Bottle Bioassay. CDC, USA: 1-83.
El-Naggar A.E., Abdalla M.S., El SebaeyA.S., Badawy S.M., 2009. Clinical Findings and Cholinesterase Levels in Children of Organophospate and Carbamate Poisoning. Europe Journal Pediatric, 168: 951-956.
Harfriani, H. 2014. Efektivitas Larvasida Ekstrak Daun Sirsak dalam Membunuh Jentik Nyamuk (Studi di Daerah Endemis DBD Kelurahan Gajahmungkur Kota Semarang). Unnes Journal of Public Health, 3(3).
Heel, V.H dan Hachimi-Idrissi, S.,2011. Accidental organophospate insecticide intoxication in children : a reminder. International Journal of Emergency Medicine, 4: 32.
Kamgang, B., Marcombe, S., Chandre, F., Nchoutpouen, E., Nwane, P., Etang, J., et al. 2011. Insecticide Susceptibility of Aedes aegypti and Aedes albopictus in Central Africa. Parasites& vectors, 4 (1): 79-86.
Khalalia, R. 2016. Uji Daya Bunuh Granul Ekstrak Limbah Tembakau (Nicotianae Tabacum L) terhadap Larva Aedes Aegypti. Unnes Journal of Public Health, 5(4): 366-374.
Lima, E.P., Paiva, M.H.S., de Araújo, A.P., da Silva, E.V.G., da Silva, U.M., de Oliveira, L.N., et al. 2011. Insecticide Resistance in Aedes aegypti Populations from Ceará, Brazil. Parasites & vectors, 4(5): 1-12.
Listyorini, P.I. 2012. Uji Keamanan Ekstrak Kayu Jati (Tectona Grandis L.F) sebagai Bio-Larvasida Aedes Aegypti Terhadap Mencit. Unnes Journal of Public Health, 1(2).
Liu N., Li M., Gong Y., Liu F., Li T. 2015. Cytochrome P450s-Their Expression, Regulation, and Role in Insecticide Resistance. Pesticide Biochemical Physiology, 120:77–81
Scott J.G., Leichter C.A., Rinkevich F.D., Harris S.A., Su C., Aberegg L.C., Moon R., Geden C.J., Gerry A.C., Taylor D.B., et al. 2013. Insecticide Resistance in House Flies From the United States: Resistance Levels and Frequency of Pyrethroid Resistance Alleles. Pesticide Biochemical Physiology, 107: 377–384.
Selvi, S.,Edah, M.A., Nazni, W.A., Lee, H.L., Tyagi, B.K., Sofian-Azirun, M., et al. 2010. Insecticide Susceptibility and Resistance Development in Malathion Selected Aedes Albopictus (Skuse). Trop Biomed, 27(3): 534-550.
Sivan, A., Shriram, A. N., Sugunan, A. P., Anwesh, M., Muruganandam, N., Kartik, C., et al. 2016. Natural Transmission of Dengue Virus Serotype 3 by Aedes albopictus (Skuese) During an Outbreak In Havelock Island: Entomological Characteristics. Acta Tropica, 156: 122-129.
Wang Q., Li M., Pan J., Di M., Liu Q., Meng F., Scott J.G., Qiu X. 2012. Diversity and frequencies of Genetic Mutations Involved in Insecticide Resistance in Field Populations of The House Fly (Musca domestica L.) from China. Pesticide Biochemical Physiology, 102:153–159.
WHO, 2009. Dengue Guidelines for Diagnosis, Treatment, Prevention and Control, WHO Library Cataloguing in Publication Data Dengue. Geneva: World Health Organization.
WHO. 2012. Global Strategy for Dengue Prevention and Control 2012–2020. Geneva: World Health Organization.