The Effect of Tamarind Fruit Nanoparticles (Tamarindus indica) on the Sperm Quality of Hyperglycemic Rats
DOI:
https://doi.org/10.15294/unnesjlifesci.v14.i2.24064Keywords:
Hyperglicemia, Nanoparticles, Tamarind Fruit, Sperm QualityAbstract
Hyperglycemia is an increase in glucose levels in the blood. This condition can trigger glucose autooxidation which can produce free radicals. Free radicals can cause damage to male reproductive organs and quality. Tamarind fruit has a high flavonoid which has the potential as an antioxidant. This study aims to analyze the effect of tamarind fruit nanoparticles on spermatozoa quality in hyperglycemic rats. This study is experimental with Post-Test Control Group Design. A sample of 15 male Wistar rats were induced by alloxan and then divided into three groups, namely the control group, tamarind fruit nanoparticles with a dose of 30 mg/KgBB (NP1) and 60 mg/KgBB (NP2) for 28 days. Sperm quality was observed with parameters of concentration, motility and viability. The data were analyzed using One Way ANOVA and LSD. The results of the Anova test showed that tamarind nanoparticle treatment could significantly improve sperm quality (P<0.05). The LSD test showed that the concentration, motility and viability of sperm in group (K) were significantly different from groups NP1 and NP2. The highest to lowest average sperm concentration in sequence was in groups NP2 (49.60x106±6.06x106), NP1 (41.60x106±7.40x106) and K (20.80x106±7.56x106). The highest to lowest average percentage of sperm motility in sequence was in groups NP2 (82.20±9.75), NP1 (74.00±4.74), K (47.20±10.66). The highest to lowest average percentage of sperm viability in sequence was in the NP2 group (76.80±4.49), NP1 (72.20±7.12), K (31.60±7.89). The conclusion of this study is that tamarind nanoparticles have an effect on improving sperm quality in hyperglycemic rats.
Downloads
References
Abdassah, M. (2017). Nanopartikel Dengan Gelasi Ionik. Jurnal Farmaka, 15(1), 45–52.
Adelati, S., Juniarto, A., & Miranti, I. (2016). Histopatologi Spermatogenesis Testis Tikus Wistar Diabetes Melitus. Jurnal Kedokteran Diponegoro, 5(4), 1760–1769. http://ejournal-s1.undip.ac.id/index.php/medico
Ali, I. A. M., Ahmed, A. Ben, & Al-Ahmed, H. I. (2023). Green synthesis and characterization of silver nanoparticles for reducing the damage to sperm parameters in diabetic compared to metformin. Scientific Reports, 13(1), 1–15. https://doi.org/10.1038/s41598-023-29412-3
Anonim. (2021). Diabetes Research and Clinical Practice. International Diabetes Federation (IDF). 102(2). https://doi.org/10.1016/j.diabres.2021.10.013
Ansari, P., Choudhury, S. T., Seidel, V., Rahman, A. B., Aziz, M. A., Richi, A. E., Rahman, A., Jafrin, U. H., Hannan, J. M. A., Wahab, Y. H. A. (2023). Therapeutic Potential of Quercetin in the Management of Type-2 Diabetes Mellitus. Life, 12, 1146. https://doi.org/10.3390/life12081146
Arundani, P., I’tishom, R., & Purwanto, B. (2021). Pemberian Ekstrak Rumput Kebar (Biophytum petersianum Klotszch) Terhadap Viabilitas Spermatozoa Mencit (Mus musculus) Diabetes Melitus. Oceana Biomedicina Journal, 4(1), 26–37. https://doi.org/10.30649/obj.v4i1.58
Asra, S., & Singh, U. P. (2024). A Brief Review on Pharmacognosy, Phytochemistry and Therapeutic Applications of Tamarindus indica. International Journal of Pharmaceutical Sciences and Medicine, 9(6), 148–171. https://doi.org/10.47760/ijpsm.2024.v09i06.013
Bulqis, A. R., Manik, E. N. G. A., & Wirasiti, N. (2020). Perbedaan Kualitas Sperma Pada Pasien Penderita Diabetes Mellitus Tipe 1 Dan 2 Di Rsud. Lamadukelleng, Sengkang, Sulawesi Selatan. Simbiosis, 8(1), 17. https://doi.org/10.24843/jsimbiosis.2020.v08.i01.p03
Couture, R., Mora, N., Al Bittar, S., Najih, M., Touaibia, M., & Martin, L. J. (2020). Luteolin Modulates Gene Expression Related To Steroidogenesis, Apoptosis, And Stress Response In Rat LC540 Tumor Leydig Cells. Cell Biology and Toxicology, 36(1), 31–49.https://doi.org/10.1007/s10565-019-09481-9
Darbandi, M., Darbandi, S., Agarawal, A., Sengupta, P., Durairajanayagam, D., Henkel, R., Sadeghi, M. R. (2018). Reactive Oxygen Species And Male Reproductive Hormones. Reproductive Biology and Endocrinology Journal, 16(87), 1-14. https://doi.org/10.1186/s12958-018-0406-2
Dludla, P. V., Mabhida, S. E., Ziqubu, K., Nkambule, B. B., Mbeje, S. E., Hanser, S., Basson, A. K., Pheiffer, C., Kengene, A. P. (2023). Pancreatic Β-Cell Dysfunction In Type 2 Diabetes: Implications Of Inflammation And Oxidative Stress. World journal of Diabetes, 14(3), 130-146. http://dx.doi.org/10.4239/wjd.v14.i3.130
Elbaz, Y. G., Moustafa, A., Ali, M. A., Desoky, G. E., Wabaidur, S. M., Iabal, A. (2024). Green Synthesized Silver Nanoparticles For The Treatment Of Diabetes And The Related Complications Of Hyperlipidemia And Oxidative Stress In Diabetic Rats. Journal Experimental Biology and Medicine. 248, 2237-2248. https://doi.org/10.1177/15353702231214258
Elgazar, A. F. (2019) The Potential Protective Roles of Tamarind Fruit Pulp Aqueous Extract Against Hyperglycemic and Testicular Toxicity in Alloxan Induced-Diabetic Male Rats. Journal of the Collage of Specific Education. 10(2), 35–58. https://doi.org/10.21608/sjse.2022.120347.1140
González, P., Lozano, P., Ros, G., & Solano, F. (2023). Hyperglycemia and Oxidative Stress: An Integral, Updated and Critical Overview of Their Metabolic Interconnections. International Journal of Molecular Sciences, 24(11). https://doi.org/10.3390/ijms24119352
Hasan, F. E., & Yunus, R. (2023). Fungsi Antioksidan dalam Menghambat Peroksidasi Lipid dan Meningkatkan Ketahanan Membran Eritrosit pada Penderita Diabetes Melitus. Health Information : Jurnal Penelitian, 15(2), e901. https://doi.org/10.36990/hijp.v15i2.901
Huang, R., Chen, J., Guo, B., Jiang, C., & Sun, W. (2024). Diabetes-Induced Male Infertility: Potential Mechanisms And Treatment Options. Molecular Medicine, 30(1). https://doi.org/10.1186/s10020-023-00771-x
Lestari., Zulkarnain., & Sijid, A.S.T. (2021). Diabetes Melitus: Review Etiologi, Patofisiologi, Gejala, Penyebab, Cara Pemeriksaan, Cara Pengobatan dan Cara Pencegahan. Prosiding Biologi Achieving the Sustainable Development Goals with Biodiversity in Confronting Climate Change, 6(8), 237-241.
Li, D., Jiang, C., Mei, G., Zhao, Y., Chen, L., Liu, J., Tang, Y., Gao, C., Yao, P. (2020). Quercetin Alleviates Ferroptosis of Pancreatic β Cells in Type 2 Diabetes. Nutrients, 12, 2954 https://doi.org/10.3390/nu12102954
Maiti, R., Karak, P., Misra, D. S., & Ghosh, D. (2017). Diabetes-Induced Testicular Dysfunction Correction By Hydromethanolic Extract of Tamarindus indica Linn. In Male Albino Rat. International Journal of Green Pharmacy, 11(4), 789–796.
Martin, L. J., & Touaibia, M. (2020). Improvement of testicular steroidogenesis using flavonoids and isoflavonoids for prevention of late-onset male hypogonadism. Antioxidants, 9(3). https://doi.org/10.3390/antiox9030237
Mustafa, H. N. (2023). Ameliorative potential of the quercetin on lead-induced testicular damage: morphohistometric and biochemical analysis. African Journal of Urology, 29(1). https://doi.org/10.1186/s12301-023-00369-z
Ojiako, O. A., Chikezie, P.C., Ogbuji, A. C. (2016). Blood glucose level and lipid profile of alloxan-induced hyperglycemic rats treated with single and combinatorial herbal formulations. Journal of Traditional and Complementary Medicine, 6 (2), 184-192. https://doi.org/10.1016/j.jtcme.2014.12.005
Osman, A. I., Zhang, Y., Farghal, M., Rashwan, A. K., Eltawel, A. S., Monaem, E. A., Mohammed, I. M. A., Badr, M. M., Ihara, I. Rooney, D. W., Yap, P. S. (2024). Synthesis Of Green Nanoparticles For Energy, Biomedical, Environmental, Agricultural, And Food Applications: A Review. Environmental Chemistry Letters, 22(1), 841-887. https://doi.org/10.1007/s10311-023-01682-3
Papadopoulou, A., Karkalousos, P., & Trapali, M. (2022). Effects of Diabetes Mellitus upon Sperm Quality Insight into Molecular Level. Journal of Diabetes Mellitus, 12(02), 75–86. https://doi.org/10.4236/jdm.2022.122008
Patabang, I., Kasim, S., & Taba, P. (2019). Sintesis Nanopartikel Perak Menggunakan Ekstrak Daun Kluwak Pangium edule Reinw Sebagai Bioreduktor dan Uji Aktivitasnya Sebagai Antioksidan. Jurnal Ilmu Alam Dan Lingkungan, 10(1), 42–50. https://doi.org/10.20956/jal.v10i1.6557
Raad, M. V., Firouzabadi, A. M., Naiki, M. T., Henkel, R., Fesahat, F. (2024). The Impact Of Mitochondrial Impairments On Sperm Function And Male Fertility: A Systematic Review. Reproductive Biology and Endocrinology Journal, 22(83), 1-17. https://doi.org/10.1186/s12958-024-01252-4
Ramos-Riera, K. P., Pérez-Severiano, F., & López-Meraz, M. L. (2023). Oxidative Stress: A Common Imbalance In Diabetes And Epilepsy. Metabolic Brain Disease, 38(3), 767–782. https://doi.org/10.1007/s11011-022-01154-7
Rifaai, R. A., El-Tahawy, N. F., Saber, E. A., Ahmed, R. (2015). Effect Of Quercetin On The Endocrine Pancreas Of The Experimentally Induced Diabetes In Male Albino Rats: A Histological And Immunohistochemical Study. Journal Diabetes Metab, 3(2) (182). https://doi.org/10.4172/2155-6156.1000182
Saputri, D. A., Christijanti, W., Lisdiana., & Iswari, R.S. (2021). Pengaruh Ekstrak Daun Kelor (Moringa oleifera) Terhadap Kualitas Sperma Tikus Hiperglikemia. Prosiding Semnas Biologi. 9, 167-171.
Taurina, W., Sari, R., Hafinur, U. C., Wahdaningsih, S., & Isnindar, I. (2017). Optimization Of Stirring Speed And Stirring Time Toward Nanoparticle Size Of Chitosan-Siam Citrus Peel (Citrus nobilis L.var Microcarpa) 70% Ethanol Extract. Majalah Obat Tradisional, 22(1), 16. https://doi.org/10.22146/tradmedj.24302
Tarmizi, M., & Siregar, F. A. (2024). Hubungan Faktor Metabolik Dan Konsumsi Makanan Minuman Manis Dengan Kadar Gula Darah Pada Usia 30-60 Tahun Di Puskesmas Simalingkar. Tropical Public Health Journal, 4(1), 27–34. https://doi.org/10.32734/trophico.v4i1.14534
Ullah, A., Khan, A., Khan, A. (2016). Diabetes Mellitus And Oxidative Stress A Concise Review. Saudi Pharmaceutical Journal. 24(5), 547-553. https://doi.org/10.1016/j.jsps.2015.03.013
Walke, G., Gaurkar, S. S., Prasad, R., Lohakare, T., & Wanjari, M. (2023). The Impact of Oxidative Stress on Male Reproductive Function: Exploring the Role of Antioxidant Supplementation. Cureus, 15(7), 1–13. https://doi.org/10.7759/cureus.42583
Wijayanti, R., & Lestari, A. P. (2018). Peningkatan Sel Sertoli Tikus Jantan Model DM Melalui Pemberian Ekstrak Parijoto (Medinilla Speciosa Blume). Pharmed: Journal of Pharmaceutical Science. 3(1), 19–28.
Yusuf, A., Almotairy, A. R. Z., Henidi, H., Alshehri, O. Y., & Aldughaim, M. S. (2023). Nanoparticles as Drug Delivery Systems : A Review of the Implication of Nanoparticles Physicochemical Properties on Responses in Biological Systems. Polymers. 15, 1596. https://doi.org/10.3390/polym15071596
Zhang, X., Tang, Y., Lu, G., & Gu, J. (2023). Pharmacological Activity of Flavonoid Quercetin and Its Therapeutic Potential in Testicular Injury. Nutrients, 15(9), 1–21. https://doi.org/10.3390/nu15092231
